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OJVRTM

Online Journal of Veterinary Research

Volume 10 (1) : 17- 19, 2006. Redacted 2018.


Effects of Doxorubicin on isolated rat ventricles enzyme activity

 

Sukullaya Assarasakorna  (DVM, MSc),  Chollada Buranakarla  (DVM, PhD),  Prasan Dhumma-Upakornb  (PhD).

 

aDepartment of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Patumwan,  bDepartment of Pharmacology, Faculty of Pharmaceutical Science, Chulalongkorn University, Henri Dunant Road, Patumwan, Bangkok, Thailand, 10330

ABSTRACT

 

Assarasakorn S,  Buranakarl C,  Dhumma-Upakorn P,  Effects of Doxorubicin on Enzyme Activities of Isolated Rat Ventricles, Onl J Vet Res., 10 (1) : 17-19, 2006. The subacute effects of doxorubicin on myosin ATPase, actomyosin ATPase, and creatine kinase were evaluated on isolated ventricles of 24 male 250-300g Wistar rats. Treated rats were injected twice intraperitoneally with 5 mg/kg doxorubicin and controls with intraperitoneal saline.  Enzymes from isolated ventricles were determined at 10 days after treatment. Myosin and actomyosin ATPase in treated animals were not different from those of control group but there was a significant reduction in creatine kinase in treated rats (P<0.05). The findings suggest that subacute h doxorubicin did not alter cardiac myosin and actomyosin ATPase, but might suppress creatine kinase.

 

Keywords : Actomyosin ATPase; Creatine kinase; Doxorubicin; Myosin ATPase; Rat.


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