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Online Journal of Veterinary Research

Volume 21(6):308-316, 2017.

Effect of hydralazine on doxorubicin-induced testicular toxicity and sperm disorders in NMRI mice


Asadollahi Negar, Azadbakht Mehri.


Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran.




Negar A, Mehri A., Effect of hydralazine on doxorubicin-induced testicular toxicity and sperm disorders in NMRI mice, Onl J Vet Res., 21(6):308-316, 2017. Groups of 8 male NMRI mice each, aged 6 to 8 weeks were given 3mg/kg intraperitoneal injection (IP) doxorubicin on days 7, 14 and 21, 5mg/kg, hydrazaline IP for 21 days and hydralazine IP 7 days before doxorubicin at days 7, 14 and 21. Controls were injected saline IP. Mice were sacrificed by cervical dislocation 28 or 64 days after the 1st treatment. Results suggested that doxorubicin reduced sperm count, viability, motility, spermatogonia, primary spermatocyte, spermatid and sertoli cells with elevated abnormal spermatozoids (P<0.05). Diameters of seminiferous tubular and germinal epithelial thickness decreased by morphometric assessment (P<0.05). In mice given hydrazaline after doxorubicin, sperm quantity and quality returned to normal. Results suggest that hydralazine may affect doxorubicin-induced testicular toxicity in mice.


Key words: Doxorubicin, Hydralazine, Testes, Sperm, Mice