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Online Journal of Veterinary Research©
Volume 21 (12):777-783, 2017.
Canine mammary tumor matrix metalloproteinase 11 catalytic domain gene identification, cloning and sequence analysis.
BV Sunil Kumar (MVSc, PhD), Meena Kataria (MSc, PhD), Ravi Kumar*(MVSc, PhD), K. Aswani Kumar (MVSc, PhD) and AP Vishwanatha Reddy (MVSc)
Enzymology lab., Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly-243122 (U.P.), India.
Sunil Kumar BV, Kataria M, Kumar R, Kumar KA, Vishwanatha Reddy AP., Canine mammary tumor matrix metalloproteinase 11 catalytic domain gene identification, cloning and sequence analysis, Onl J Vet Res., 21 Volume 21 (12):777-783, 2017. Matrix metalloproteinases (MMP) are the main group of proteolytic enzymes involved in tumor invasion, metastasis, and angiogenesis in cancer. We describe a partial sequence of canine MMP11 catalytic domain cloned and sequenced to Accession number HM037636. Upon phylogenetic analysis, the nucleotide sequence was found close to Equus caballus while it clustered away from rodent and primate groups. The translated protein sequence also showed a similar trend. The synonymous substitution was higher than the non-synonymous one suggesting that catalytic domain MMP11 gene was not under positive selection, and advantageous mutations would not have an important role in evolutionary adaptation.
Key words: Cloning, Characterization, Matrix metalloproteinase 11, canine.