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OJVR
Online Journal of Veterinary Research©
Volume 21 (12):777-783, 2017.
Canine mammary tumor matrix metalloproteinase 11
catalytic domain gene identification, cloning and sequence analysis.
BV Sunil Kumar (MVSc, PhD), Meena Kataria (MSc, PhD), Ravi Kumar*(MVSc,
PhD), K. Aswani Kumar (MVSc,
PhD) and AP Vishwanatha Reddy (MVSc)
Enzymology lab.,
Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly-243122 (U.P.), India.
ABSTRACT
Sunil Kumar
BV, Kataria M, Kumar R, Kumar KA, Vishwanatha
Reddy AP., Canine mammary tumor matrix metalloproteinase 11 catalytic domain gene identification, cloning
and sequence analysis, Onl J Vet Res., 21 Volume 21 (12):777-783, 2017. Matrix
metalloproteinases (MMP) are the main group of proteolytic enzymes involved in tumor
invasion, metastasis, and angiogenesis in cancer. We describe a partial
sequence of canine MMP11 catalytic domain cloned and sequenced to Accession
number HM037636. Upon phylogenetic analysis, the nucleotide sequence was found
close to Equus caballus
while it clustered away from rodent and primate groups. The translated protein
sequence also showed a similar trend. The synonymous substitution was higher
than the non-synonymous one suggesting that catalytic domain MMP11 gene was not
under positive selection, and advantageous mutations would not have an
important role in evolutionary adaptation.
Key words: Cloning, Characterization,
Matrix metalloproteinase 11, canine.
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