©1996-2018. All Rights
Reserved. Online Journal of Veterinary Research. You
may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJVR. This article may be
copied once but may not be, reproduced or re-transmitted without the express
permission of the editors. This journal satisfies the refereeing requirements
(DEST) for the Higher Education Research Data Collection (Australia). Linking:
To link to this page or any pages linking to this page you must link directly
to this page only here rather than put up your own page
OJVRTM
Online Journal of
Veterinary Research©
Volume (9):898-903, 2019.
Effect of adipose-derived osteogenic mesenchymal stem cells on
severe osteoarthritis lameness in dogs.
Patrícia Furtado Malard¹ (DVM, PhD),
Hilana dos Santos Sena
Brunel¹ (DVM, PhD), Paulo Henrique Sampaio da Silva²
(DVM),
Cecília Ferreira Amaral Silva²(DVM), Lara Regina
Pontes Cury ²(DVM), André Rodrigues da Cunha Barreto Vianna³(DVM, PhD),
Eduardo Maurício
Mendes de Lima (DVM,
PhD)²*.
¹Biocell Lab - Cellular
Therapy, SMPW Qd.05, Brasília, DF 71735-500,²Faculty of Agronomy and Veterinary Medicine, University of
Brasília (UnB), ICC Sul, Campus Universitário Darcy Ribeiro, Cx. Postal, 4508,
Brasília, DF 70910-970. ³Department of Animal Anatomy , Federal University of
Paraná – Palotina Sector (UFPR), R. Pioneiro, 2153 - Dallas, Palotina - PR,
85950-000 *limaemm@hotmail.com
ABSTRACT
Malard PF, Santos Sena HB, Sampaio
da Silva PH, Amaral Silva CF, Pontes Cury LR, Barreto Vianna AR, Mendes de Lima EM. Effect of adipose-derived mesenchymal stem
cells on severe osteoarthritis lameness in dogs, Onl
J Vet Res., (9):898-903, 2019. We evaluated adipose-derived mesenchymal stem cells for treatment of severe osteoarthritis
lameness in 8 dogs, without anti-inflammatory drugs.
Under anesthesia, we excised adipose tissue from the
subcutaneous gluteal region, above the greater trochanter, corresponding to 1cm3
from each dog. Stem cells were identified after 72h incubation and flow cytometry for
expression of hematopoietic cell markers, CD45 CD34, CD90 and CD29. After 21
days of self-cultivation, cells differentiated into osteogenic lineage cells
confirmed by Von Kossa stain, microscopy and
mineralization. Dogs were evaluated for lameness with 7 being severe at Day 0, and 1 dog having a
milder form. Dogs were then injected 2 million ± 10%
cells per affected intra-articular
joint and evaluated for lameness at 30 and 180 days after
treatment. At 30 or 180 days
we observed visible reduction in lameness in all dogs, one with complete
remission at Day 30. By 180 days, we found complete remission in another 2 dogs
which had had severe lameness. Our results suggest that adipose derived mesenchymal stem cells could be used to treat
osteoarthritis with 3 dogs showing complete
remission. Further research with larger cohorts of dogs and imaging of joints
may confirm these findings.
Keywords: Mesenchymal cells , Adipose, Osteoarthritis,
Lameness, Dogs.
FULL-TEXT
(SUBSCRIPTION OR PURCHASE TITLE)