©1994-2018. All Rights
Reserved. Online Journal of Veterinary Research. You
may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJVR publications. This
article may be copied once but may not be, reproduced or re-transmitted without
the express permission of the editors.
OJVRTM
Online Journal of Veterinary Research©
Volume 10 (2) : 219-234, 2006. Extensively redacted 2018.
Diagnostics for Brucella melitensis in Baladi does.
Abd El-Razik
KA, Desouky HM, Ahmed WM
Department of Animal Reproduction & A.I., National
Research Center, Dokki, Giza, Egypt
ABSTRACT
Abd El-Razik KA, Desouky
HM, Ahmed WM, Diagnostics for Brucella melitensis
in Baladi does, Onl J Vet Res., 10 (2) 219-234, 2006. Diagnostic methods for early detection of brucellosis (Br. melitensis)
were evaluated in does. Ten mature pregnant Baladi
does were used as controls or
inoculated intra-conjunctivally with Br. melitensis biovar 3 between
40 and 45 days of gestation. Blood
samples were collected weekly for immunological tests. Does with abortions were
necropsied together with the rest of animals, 3 months later for
bacteriological, PCR and pathological investigations. Results indicated that does
inoculated with Br. melitensis showed severe
respiratory disorders and abortion, 3 – 5 weeks post inoculation. Br. melitensis was isolated from
the spleen, and lymph nodes from all inoculated does. Indirect Enzyme Linked Immunosorbent Assay (ELISA), Buffered Acidified Plate Antigen Test (BAPAT) and Rose Bengal Plate Antigen Test (RBPT) showed very early detection of
infection in the serum (4-7th days post inoculation, DPI). Rivanol Test (RVT) showed relatively early detection (7th DPI) while, Complement Fixation Test (CFT) and Tube Agglutination Test (TAT) showed late
detection of antibody titer (begins 14th DPI). A significant
lymphocyte transformation response among the different SBP50 concentrations was
noticed in inoculated does. All tissues specimens taken from inoculated does
yielded a positive PCR product specific for brucella.
The histopathological examination revealed diffuse suppurative
and necrotizing placentitis, ulcerative endometritis
and presence of large colonies of brucellae, either
extra or intracellularly. It could be concluded that for diagnosis of
brucellosis in goats, RBPT should be combined with iELISA
as screening tests, while CFT and /or PCR could be used as a confirmatory test.
Detecting colonies of brucellae (extra-or intra
cellular) in the placental tissue of aborted does is the most specific
pathological lesion.
Key words:
goats – Brucella melitensis - serology - PCR – pathology
FULL-TEXT (SUBSCRIPTION OR PURCHASE TITLE $25USD)