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OJVRTM
Online Journal of Veterinary Research©
Volume 23 (9):914-925,
2019.
Genetic diversity of sheep
in Iraq.
Wafaa S Al-Sabea1, Drgham H Y Al- Zwean2, Mohammad A Al- Faham3
1Sheep Section,
Agriculture Research Directorate, Ministry of Science and
Technology, 2Faculty of Veterinary
Medicine, University of Baghdad, 3Faculty
of Medicine, University of Baghdad, Iraq.
Al-Sabea
WS, Al- Zwean DHY, Al- Faham
MA., Genetic diversity of sheep in Iraq, Onl J Vet
Res. 23 (9) 914-925, 2019. We report genetic diversity by
PCR in Awasi, Naimi, Hamdani and mixed breed
sheep on 10 short tandem repeat microsatellite markers. Genetic analysis
revealed high allelic and gene diversity in all sheep. We detected 162 alleles with 10 microsatellite
loci with a mean of 9.175 and effective loci of 5.760. Maximum alleles (28) were found in marker
ILSTS5 and minimum in MAF33 (8). Expected
heterozygosity was 0.778 and observed 0.797 suggesting that the microsatellite markers were suitable for genotyping.
Within population estimates (FIS: -0.011)
suggested a low rate of inbreeding. Genotypic
genetic distance from Pairwise population matrix of mean populations was
plotted showing that Hamdani originated first from
the root and the highest genetic distance was observed
between Hamdani
and Awassi
sheep.
Key words: Genetic diversity, Microsatellite, DNA , sheep, Iraq.
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