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Online Journal of Veterinary Research©
Volume 23 (9):914-925, 2019.
Genetic diversity of sheep in Iraq.
Wafaa S Al-Sabea1, Drgham H Y Al- Zwean2, Mohammad A Al- Faham3
1Sheep Section, Agriculture Research Directorate, Ministry of Science and Technology, 2Faculty of Veterinary Medicine, University of Baghdad, 3Faculty of Medicine, University of Baghdad, Iraq.
Al-Sabea WS, Al- Zwean DHY, Al- Faham MA., Genetic diversity of sheep in Iraq, Onl J Vet Res. 23 (9) 914-925, 2019. We report genetic diversity by PCR in Awasi, Naimi, Hamdani and mixed breed sheep on 10 short tandem repeat microsatellite markers. Genetic analysis revealed high allelic and gene diversity in all sheep. We detected 162 alleles with 10 microsatellite loci with a mean of 9.175 and effective loci of 5.760. Maximum alleles (28) were found in marker ILSTS5 and minimum in MAF33 (8). Expected heterozygosity was 0.778 and observed 0.797 suggesting that the microsatellite markers were suitable for genotyping. Within population estimates (FIS: -0.011) suggested a low rate of inbreeding. Genotypic genetic distance from Pairwise population matrix of mean populations was plotted showing that Hamdani originated first from the root and the highest genetic distance was observed between Hamdani and Awassi sheep.
Key words: Genetic diversity, Microsatellite, DNA , sheep, Iraq.