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Online Journal of Veterinary Research


Volume 23 (8):786-798, 2019.

Genetic diversity of White Leghorns versus brown egg birds by multi-locus DNA (RAPD) markers.


Mahadeo Kumar MVSc1, SK Mishra PhD2, VLK Prasad PhD3, RP Sharma PhD4 and BR Gupta PhD5.


1Indian Institute of Toxicology Research, Lucknow, 2Central Avian Research Institute, Izatnagar, Bareilly, 3Department of Livestock Production and Management, College of Veterinary Science, Rajendranagar Hyderabad, 4Project Directorate on Poultry, Hyderabad, India, 5Department of Animal Genetics and Breeding, College of Veterinary Science, Hyderabad, India.



Kumar M, Mishra SK, Prasad VLK, Sharma RP, Gupta BR, Genetic diversity of White Leghorn versus brown egg birds by multi-locus DNA (RAPD) markers, Onl J Vet Res., 23 (8):786-798, 2019. A study was undertaken to analyze genetic diversity of 5 egg-type closed chicken populations, viz. 4 White Leghorn (WLH) strains: IWI, IWH, IWF and IWD which had undergone selection for more than 20 generations, and one brown-egger strain: D. Red with variant geographic origin. A cumulating total of 145 randomly amplified polymorphic DNA (RAPD) markers generated from 10 most polymorphic primers, screened out of 42 were employed. The WLH strains exhibited distinct inter-population genetic differences as correlated to their origin and subsequent selection history. The intra-population genetic similarity ranged from 0.8484 to 0.9011 where as inter-population genetic similarity ranged from 0.5682 to 0.8854. The coefficient of population-genetic differentiation (GST) was 0.422 which indicated higher contribution of within-population differences to genetic variation. A combined Dendrogram incorporating all the above strains in form of un-rooted tree was constructed, which revealed that the D. Red clustered distinctly away from the WLH strains, while displaying the highest genetic distance from IWD (0.5645). Within the WLH strains, the IWI and IWD were at maximum genetic distance (0.23) while the least (0.122) distance was recorded between IWF and IWD. It was concluded that differential genetic-homogeneity and distinct clustering patterns within egg type chicken strains consistent to their breeding and selection histories could be efficiently delineated by RAPD markers.


Key words: Genetic diversity, polymorphism, RAPD, Long-term selection and WLH.