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OJVRTM
Online Journal of Veterinary Research©
Volume
23 (8):786-798, 2019.
Genetic
diversity of White Leghorns versus brown egg birds by multi-locus DNA (RAPD)
markers.
Mahadeo Kumar MVSc1, SK Mishra PhD2,
VLK Prasad PhD3, RP Sharma PhD4 and BR Gupta PhD5.
1Indian Institute of
Toxicology Research, Lucknow, 2Central Avian Research Institute, Izatnagar, Bareilly, 3Department
of Livestock Production and Management, College of Veterinary Science, Rajendranagar Hyderabad, 4Project Directorate on Poultry, Hyderabad, India, 5Department
of Animal Genetics and Breeding, College of Veterinary Science, Hyderabad,
India.
ABSTRACT
Kumar M, Mishra SK,
Prasad VLK, Sharma RP, Gupta BR, Genetic diversity of White Leghorn versus brown egg birds by multi-locus
DNA (RAPD) markers, Onl J Vet Res., 23 (8):786-798, 2019. A study was
undertaken to analyze genetic diversity of 5 egg-type closed chicken
populations, viz. 4 White Leghorn (WLH) strains: IWI, IWH, IWF and IWD which
had undergone selection for more than 20 generations, and one brown-egger
strain: D. Red with variant geographic origin. A cumulating total of 145 randomly
amplified polymorphic DNA (RAPD) markers generated from 10 most polymorphic
primers, screened out of 42 were employed. The WLH strains exhibited distinct inter-population
genetic differences as correlated to their origin and subsequent selection
history. The intra-population genetic similarity ranged from 0.8484 to 0.9011 where as inter-population genetic similarity ranged from
0.5682 to 0.8854. The coefficient of
population-genetic differentiation (GST) was 0.422
which indicated higher contribution of within-population differences to genetic
variation. A combined Dendrogram incorporating all
the above strains in form of un-rooted tree was constructed, which revealed
that the D. Red clustered distinctly away from the WLH strains, while displaying
the highest genetic distance from IWD (0.5645). Within the WLH strains, the IWI
and IWD were at maximum genetic distance (0.23) while the least (0.122)
distance was recorded between IWF and IWD. It was concluded that differential
genetic-homogeneity and distinct clustering patterns within egg type chicken
strains consistent to their breeding and selection histories could be efficiently
delineated by RAPD markers.
Key words: Genetic diversity, polymorphism, RAPD,
Long-term selection and WLH.
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