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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 25 (9):666-671, 2021.
Detection of canine distemper virus in conjunctival, genital, urine and blood samples
by hemi-nested PCR in dogs with neurological signs.
Amaral1 HA, Cortez1 A, Richtzenhain1 LJ, Funada2 MR, Soares1 RM, Durigon2 EL, Larsson1 MHMA.
1School of Veterinary Medicine and Animal Science, University of São Paulo, Brazil; 2Institute of Biomedical Sciences, University of São Paulo, Brazil.
Amaral H, Cortez A, Richtzenhain LJ, Funada MR, Soares RM, Durigon EL, Larsson MHMA. Detection of canine distemper virus in conjunctival, genital, urine and blood samples by hemi-nested PCR in dogs with neurological signs, Onl J Vet Res., 25 (9):666-671, 2021. Canine distemper is highly contagious inducing severe neurological signs in dogs. We report incidence of virus in conjunctival and genital swabs, urine and peripheral blood mononuclear cells by hemi-nested RT-PCR taken from 50 dogs with neurological signs presented at the Veterinary Hospital, School of Veterinary Medicine and Animal Science, University of São Paulo, over 3 years. Only unvaccinated dogs or those vaccinated 50 days before sampling were tested to exclude false-positives due to vaccine virus. Fragments of nucleoprotein genes canine distemper virus were detected by hemi-nested PCR in 43 (86%) dogs. We found 40 (80%) in genital, 37 (74%) in conjunctiva swabs, 37 (74%) from urine and 33 (68%) in peripheral blood mononuclear cells. We find that conjunctival and genital swabs, and urine samples provided high detection sensitivity and convenient sampling. Detection was boosted by testing two samples especially in dogs without extra-neural signs or convalescent late stage of canine distemper.
Key words: canine distemper; conjuctival swab; dogs; genital swab; hemi-nested-PCR; RT-PCR; urine.