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OJVRTM
Online Journal of
Veterinary Research©
Volume 12 (2):70-75, 2008. Slightly Redacted 2018.
PCR detection
of canine distemper virus in biological fluids
Amaral HA1,
Cortez A1, Richtzenhain LJ1 ,
Funada MR2, Soares
RM1, Durigon EL2, Larsson MHMA1.
1School of Veterinary Medicine and Animal Science and
2Institute of Biomedical Sciences, University of São Paulo, Brazil.
ABSTRACT
Amaral HA; Cortez A; Richtzenhain
LJ; Funada MR; Soares
RM; Durigon EL; Larsson MHMA., PCR
detection of canine distemper virus in biological fluids, Onl
J Vet Res., 12 (2):70-75, 2008. Canine
distemper is a highly contagious disease affecting dogs worldwide, often
inducing severe neurological signs. We used RT-PCR to detect distemper virus in
conjunctival and genital swabs, urine and peripheral blood mononuclear cells
taken from 50 dogs with neurological signs. Only dogs that had not been
vaccinated or that were vaccinated more than 50 days before the samples were
collected were included to exclude any false-positive results from vaccine
virus. Fragments of nucleoprotein gene canine distemper virus were detected by
hemi-nested PCR in 43 (86%) genital swabs, 40 (80%) conjunctival swabs, 37
(74%) urine and 33 (68%) peripheral blood mononuclear cells. Conjunctival and
genital swabs, and urine provided high detection
sensitivity, convenient sample processing and less risk to the animals.
Sensitivity for detecting positive results was increased by testing 2 samples
(genital or conjunctival swab and urine sample), especially in dogs that did
not exhibit extra-neural signs or those in convalesce or late stage of canine
distemper.
Key words: canine distemper; conjuctival swab;
dogs; genital swab; hemi-nested-PCR; RT-PCR; urine.
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