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Online Journal of Bioinformatics ©
Volume 13(3):390-398, 2012.
Glycosyltransferase
sequence informatics from different source organisms
Vivek Dhar Dwivedi¹, Amit Kumar
Pandey², Sarad Kumar Mishra³*
¹Departments of Bioinformatics and ² Forest Pathology, Dehradun and ³Department of Biotechnology, DDU
Gorakhpur University, Gorakhpur.
Abstract
Dwivedi VD, Pandey AK, Mishra SK., Glycosyltransferase sequence informatics from different
source organisms, Online J Bioinform., 13(3):390-398,
2012.
Thirty full- length amino acid sequences of glycosyltransferase
from fungi, plants and animals were collected and subjected to multiple
sequence alignment (MSA), motif identification, domain identification, amino acid
composition, and phylogenetic tree construction. MSA revealed that a single tryptophan residue was a conserved
substitution in all plant and animal sequences profile, a single tryptophan
residue was also conserved substitution in all plant and fungi sequences
profile and no any conserved residue was found in animal and fungi sequences
profile. Three major sequence clusters were constructed by phylogenetic
analysis. Cluster I contained 7 species of plant, 5 of fungi, and 2 of animals,
Cluster II had 3 species of plants, 3 of animals, and 2 of fungi, whereas Cluster III had 5 of animals and 3 of fungi.
Amino acid composition result revealed that the average frequency of amino acid
leucine was 9.84 percent which was high compared with
other amino acids. The average frequency of cysteine was 1.79 which was low compared with analyzed species.
Nine motifs which were unique for their groups were also identified.
Keywords: Glycosyltransferase. Phylogenetic analysis.
Conserved regions. Motifs. Domains. Amino acid composition.