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OJVRTM

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

Volume 27 (3): 175-183, 2023.

Dextran conjugated lysozyme milk antibacterial.

 

Safari J1, Aminlari L2, Aminlari M1,3

 

Department(s) of 1Food Science and Technology, 2Public Health, 3Biochemistry, College of Agriculture, School of Veterinary Medicine, Shiraz University Iran.‎

 

ABSTRACT

 

Safari J, Aminlari L, Aminlari M., Dextran conjugated lysozyme milk antibacterial, Onl J Vet Res., 27 (3): 175-183, 2023. Lysozyme is effective against G-positive bacteria but not against ‎G-negatives due to liposaccharide surface. We conjugated lysozyme with the ‎polysaccharide dextran by Maillard reaction and determined its effect on G-positive and negative bacteria in milk by reaction at 60◦C for 7 days. Glycosylated yields were separated by chromatography confirmed by electrophoresis and tested against S. aureus and E. coli  in culture and milk‎. Results suggested that 250 µg/ml, lysozyme–dextran conjugate decreased S. aureus and E. coli 1.5 and ‎‎2.4 log, and 400 µg/ml 2 and 4 log.

 

Key words: Lysozyme, dextran, milk, S. aureus, E. coli.

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