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Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X


Volume 27 (3): 175-183, 2023.

Dextran conjugated lysozyme milk antibacterial.


Safari J1, Aminlari L2, Aminlari M1,3


Department(s) of 1Food Science and Technology, 2Public Health, 3Biochemistry, College of Agriculture, School of Veterinary Medicine, Shiraz University Iran.




Safari J, Aminlari L, Aminlari M., Dextran conjugated lysozyme milk antibacterial, Onl J Vet Res., 27 (3): 175-183, 2023. Lysozyme is effective against G-positive bacteria but not against G-negatives due to liposaccharide surface. We conjugated lysozyme with the polysaccharide dextran by Maillard reaction and determined its effect on G-positive and negative bacteria in milk by reaction at 60C for 7 days. Glycosylated yields were separated by chromatography confirmed by electrophoresis and tested against S. aureus and E. coli  in culture and milk. Results suggested that 250 µg/ml, lysozyme–dextran conjugate decreased S. aureus and E. coli 1.5 and ‎‎2.4 log, and 400 µg/ml 2 and 4 log.


Key words: Lysozyme, dextran, milk, S. aureus, E. coli.