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OJVRTM
Online
Journal of Veterinary Research©
Volume 18(7): 543-553, 2014. Redacted 2017.
Antimicrobial
effect of lysozyme dextran conjugate on E. coli
and S. aureus in milk.
Safari J1, Aminlari L2, Aminlari
M1,3*
1Department(s)
of Food Science and technology, 2Food Hygiene and Public Health, 3Biochemistry, College
of Agriculture, School of Veterinary Medicine, Shiraz
University, Iran
ABSTRACT
Safari J, Aminlari L, Aminlari M.,
Antimicrobial effect of lysozyme dextran conjugate on E. coli and S. aureus in milk.
Onl J Vet Res., 18(7): 543-553,
2014. Lysozyme is effective
against G-positive bacteria but not G-negative bacteria.
Antimicrobial effects of a lysozyme dextran conjugate against S. aureus
and E. coli in culture media and milk are described. Lysozyme and dextran were reacted at 60◦C
for 7 days. Glycosylated products were separated by CM-25 cation-exchange
chromatography and confirmed by electrophoresis. At 250 µg/ml
the conjugate decreased S. aureus and E. coli by 1.5 and 2.4 log cycles respectively, after 48 hr
incubation, and at 400 µg/ml, by 2 and 4 log cycles. In sterilized milk, native lysozyme did not
affect E. Coli
counts but S. aureus decreased by 0.5 log cycle. The conjugate decreased E. coli and S. aureus by 2.3 and
0.4 log cycles.
Key words: Lysozyme, dextran, conjugation, maillard,
antimicrobial, Gram negative.
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