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Online Journal of Veterinary Research

Volume 24 (4):224-229, 2020.

Serum mineral, biochemical and hormone reference values

for each trimester and diagnosis of pregnancy in buffaloes


AL-Sariy SM1, AL-Yasiri EA2, AL-Hamedawi TM2


1Department(s) of Surgery and Obstetrics , College(s) of Veterinary Medicine, AL-Qasim Green University, 2University of Baghdad, Iraq.


AL-Sariy SM, AL-Yasiri EA, AL-Hamedawi TM., Serum mineral, biochemical and hormone reference values for each trimester and diagnosis of pregnancy in buffaloes, Onl J Vet Res., 24 (4):224-229, 2020. Authors report reference serum calcium (Ca+), potassium (K+), phosphorus (Po), glucose, protein urea, cholesterol, progesterone, pregnancy associated protein (PAG), prolactin and 17-estradiol levels for 1st, 2nd and 3rd trimesters of pregnancy in groups of 10 buffalo each. Compared with non-pregnant (n=10) controls, serum Po was ~42% higher (P < 0.01) during 1st but 41% lower at 3rd trimester, Ca 41% higher during 2nd but 16% lower at 3rd trimesters. We found no differences with serum K+ levels between trimesters. Cholesterol levels were highest (~9%) at 1st and 2nd but lower (~40%) during the 3rd trimester, glucose was 8% higher during the 1st but lower (16%) during the 3rd. Urea increased ~8-48% from 1st to 2nd and 3rd (~39%) and protein increased ~22-32% but only during the 1st and 3rd with no apparent change during the 2nd. We recorded very large differences in serum hormones finding 11 to 46 fold increases in progesterone, 12 to 142 with PAG, 1,6 to 3.7 with prolactin but 3.2 -3.7 fold declines in 17-Estradiol during 2nd-3rd trimesters with a slight elevation (24%) during the 2nd. Our findings suggest that elevated serum PAG may be useful for diagnosing pregnancy during 1st to 3rd trimesters in buffalo.


Keywords: buffaloes, Bio-chemical, PAG, pregnancy detection .