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OJVRTM

Online Journal of Veterinary Research©

Volume 19(6): 424-435, 2015.


Effect of C5aR antagonist on histopathological and blood parameters in female BALB mice

following chlorhexidine-induced contact dermatitis.

 

Farhana BN BSc1, Siong JJ DVM, Noordin MM DVM PhD2 and Hezmee MNM DVM PhD1

 

1Preclinical Sciences, 2Veterinary Pathology and Microbiology,  Faculty of Veterinary Medicine, Universiti Putra Malaysia Selangor, Malaysia.

 

ABSTRACT

 

Farhana BN, Siong JJ, Noordin MM, Hezmee MNM, Effect of C5aR antagonist on histopathological and blood parameters in mice following chlorhexidine-induced contact dermatitis in mice, Onl J Vet Res., 19(6): 424-435, 2015. Chlorhexidine is a commonly used antiseptic agent which can induce hypersensitivity in humans. Whether chlorhexidine produces hypersensitivity in mice is unclear. Four groups of 6 mice each were injected  dexamethasone, histamil, complement component C5a receptor antagonist PMX205, or saline (controls). Blood and skin samples were taken, and serum IgE for allergy reaction was detected by ELISA. Chlorhexidine induced contact dermatitis in all mice. Results suggest C5aR antagonist can reduce contact dermatitis by chlorhexidine compared with anti-histamine and corticosteroids. Blood parameters did not show significant changes compared with controls in any group. C5aR antagonists may have an effect on chlorhexidine-induced contact dermatitis in mice but microscopy assessments should be interpreted with caution as only 2 mice from each group were assessed.

 

Keywords: chlorhexidine, chlorhexidine-induced contact dermatitis, C5aR antagonist


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