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OJVRTM
Online Journal of Veterinary Research©
Volume 16
(3):133-137, 2012. Redacted 2017.
Detection of anti-Cysticercus
tenuicollis antibody by counter immune-electrophoresis
in experimentally infected sheep.
Masoud Kakaei1, Mohammad Hossein Razi Jalali1, Masoud
Ghorbanpoor1, Zahra Asadollahi1, Alireza
Sazmand2
1Depertment of Pathobiology, School of
Veterinary Medicine, Shahid Chamran
University of Ahvaz, Iran, 2Institute of Applied-Scientific
Education of Jihad-e-Agriculture, MolaSadra Center,
University of Applied Science and Technology, Yazd, Iran
Abstract
Masoud K, Mohammad HRJ, Ghorbanpoor
M, Asadollahi Z, Sazmand
A., Detection of anti-Cysticercus tenuicollis antibody
by counterimmunoelectrophoresis in experimentally
infected sheep, Onl J Vet Res., 16 (3):133-137, 2012. A counterimmunoelectrophoresis (CIEP) method for detection of antibodies against
sheep cysticercosis is described. Cysticercus
tenuicollis parasites were collected from
slaughtered infected sheep in a local abattoir. Two puppies were given 15
cysts, and 2 months later were autopsied after euthanasia. Taenia
hydatigena worms were collected from the small
intestine of dogs and the gravid proglottids were
crushed to release eggs. About 5000 eggs were orally administered to 4 sheep,
while another 4 were kept as controls. Sera from experimentally infected sheep
were obtained weekly up to nine weeks after infection. All serum samples were
then examined by the CIEP method. The sensitivity of the test was nil
during the first two weeks, but increased to 75% in the third week and reached
100% from the fourth week after infection to the end of the study period (9th
week). The specificity of the test was 100% in detection of C. tenuicollis antibodies throughout the experiment. Results of the present study show that CIEP test
could be recommended for ante-mortem diagnosis of natural T. hydatigena cysticercosis
using purified antigen.
Key
words: Cysticercus
tenuicollis, Counterimmunoelecterophoresis
test, Taenia hydatigena,
Sheep
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