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Online Journal of Veterinary Research©

Established 1994 
ISSN 1328-925X


Volume 22 (9):799-805, 2018.

A C57BL/6 mouse model for atopic dermatitis in dogs induced by Dermatophagoides farinae.


Talita de Cássia Borges1 DVM MSc, Cecília Ferreira Amaral Silva1 DVM,

Paulo Henrique de Sampaio da Silva1 DVM MSc, Márcio Botelho de Castro1 DVM PhD,

Andre Rodrigues da Cunha Barreto Vianna2 DVM PhD, Eduardo Maurício Mendes de Lima1 DVM PhD.


1Faculty of Agronomy and Veterinary Medicine, University of Brasília (UnB), ICC Sul, Campus Universitário Darcy Ribeiro, Cx. Postal, 4508, Brasília, DF 70910-970. 2Department of Veterinary Medicine, Federal University of Lavras (UFLA), Campus Universitário, 37.200-000 Lavras, MG, Brazil (UFLA), Minas Gerais, Brazil.

e-mail: limaemm@unb.br



Borges TC, Amaral Silva CF, Sampaio da Silva PH, Castro MB, Cunha Barreto Vianna AR, Mendes de Lima EM., A C57BL/6 mouse model for atopic dermatitis induced by Dermatophagoides farinae mites in dogs, Onl J Vet Res.,. 22 (9):799-805, 2018. Atopic dermatitis (AD) with itching and inflammation is the most common skin allergy in dogs.  We describe a mice model for AD. Three groups of 3 C57B1/6 mice were infested twice on skin with a dressing embedded with 0.1ml extract of Dermatophagoides farinae mites in 2, 3 or 4 cycles. Mice were infested on days 0, 3, 13 and 16 and rested between days 6 and 13. Controls were taped the same dressing with saline only. We only observed erythema, excoriation and pruritus in mice infested 4 cycles. In those infested 4 cycles we found hyperplasia (controls 6.03 ± 0.37mm), (infested 17.17 ± 3.93mm) and increased mast cells (controls 26 ± 1.04) (infested 137 ± 15.29). However, we found increased perivascular infiltrate in all mice infested by Dermatophagoides farinae. The C57BL/6 mice model appears to simulate canine atopic dermatitis using repeat dermal infestations with Dermatophagoides farinae.


Key words: murine model, C57Bl6, canine atopy, allergy..