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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 22 (12):1095-1102, 2018.
Prevalence and strains of Cryptosporidium detected by microscopy and PCR in stools of cattle handlers.
Haider Hussein Obed Alseady, May Hameed Kawan.
Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Iraq.
Obed Alseady HH, Kawan MH., Prevalence and strains of Cryptosporidium detected by microscopy and PCR in stools of cattle handlers, Onl J Vet Res., 22 (12):1095-1102, 2018. Authors report prevalence and species of Cryptosporidium in stools of 100 volunteer cattle handlers aged <10 - to >40 years determined by microscopy and nPCR, collected during January to September 2018 in Iraq. We found oocysts in 17% by microscopy, but by PCR detected Cryptosporidium in 41% of stool samples. By PCR, children aged < 10 had a very high incidence of 69.9% (P≤0.05), 19-20 year old ~30%, and those over 40 years ~18%. There was no significant difference in presence of Cryptosporidium between genders. By PCR with 18S rRNA gene of 10 stool samples phylogenetic tree of our C. parvum, C. hominis and C. andersoni isolates referenced against Gen-Bank revealed 5 closely related to Cryptosporidium andersoni (KX710086.1) 3 to Cryptosporidium hominis (KT123173.1), and 2 to Cryptosporidium parvum isolates (MH341586.1) with a genetic difference of 0.01-0.04%. Datasets suggested strong genetic distinctiveness amongst species. C. andersoni has not been reported previously in this region in dairy/cattle handlers but C. parvum causes most infection.
Key words: Cryptosporidium, prevalence, cattle/cow handlers, age, sex, oocytes, nested-PCR, 18S rRNA gen.