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OJVRTM
Online Journal of Veterinary
Research©
(Including Medical and Laboratory
Research)
Established 1994
ISSN 1328-925X
Volume 22 (12):1095-1102, 2018.
Prevalence and strains of Cryptosporidium
detected by microscopy and PCR in stools of cattle handlers.
Haider
Hussein Obed Alseady, May Hameed Kawan.
Department of Parasitology, College of
Veterinary Medicine, University of Baghdad, Iraq.
ABSTRACT
Obed Alseady HH, Kawan MH., Prevalence and strains
of Cryptosporidium detected by microscopy and PCR in stools of cattle handlers,
Onl J Vet Res., 22
(12):1095-1102, 2018. Authors report prevalence and species of Cryptosporidium in
stools of 100 volunteer cattle handlers aged <10 - to >40 years determined by
microscopy and nPCR, collected during January to
September 2018 in Iraq. We found oocysts in 17% by microscopy, but by PCR
detected Cryptosporidium in 41% of stool samples. By PCR, children aged
< 10 had a very high incidence of 69.9% (P≤0.05), 19-20 year old ~30%,
and those over 40 years ~18%. There was no significant difference in presence
of Cryptosporidium between genders. By PCR with 18S rRNA
gene of 10 stool samples phylogenetic tree of our C. parvum,
C. hominis and C. andersoni
isolates referenced against
Gen-Bank revealed 5 closely related to Cryptosporidium andersoni
(KX710086.1) 3 to Cryptosporidium hominis
(KT123173.1), and 2 to Cryptosporidium parvum
isolates (MH341586.1) with a genetic difference of 0.01-0.04%. Datasets
suggested strong genetic distinctiveness amongst species. C. andersoni has not been reported previously in this region
in dairy/cattle handlers but C. parvum causes
most infection.
Key words: Cryptosporidium,
prevalence, cattle/cow handlers, age, sex, oocytes, nested-PCR, 18S rRNA gen.
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