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OJVRTM

Online Journal of Veterinary Research

Volume 23 (6):568-577, 2019.


Cryptosporidium strains isolated from goat feces by PCR identified by phylogenetic tree.

 

Zeena A Alani, Amer M Al-Amery.

 

Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Baghdad, lraq.

 

ABSTRACT

 

Alani ZA, Al-Amery AM., Cryptosporidium strains isolated from goat feces by PCR identified by phylogenetic tree, Onl J Vet Res., 23 (6):568-577, 2019. We identified Cryptosporidium Spp. isolated from feces of 100 goats located in Baghdad, Iraq by PCR and phylogenetic analysis. We found 53% samples infected. We sequenced from PCR positive DNA samples using 18S nucleotide rRNA gene confirmed against NCBI-BLAST for Cryptosporidium. Results confirmed C. parvum, C. hominis and C. andersoni.

 

 


 


Keywords: Cryptosporidium, Phylogenetic Tree, PCR, Goat, Baghdad.


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