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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

Volume 27 (10):570-582, 2023.

In vivo papyrus protein and crab shell bone scaffold in dogs.


Khayreia Kadhim Habeeb1, Bahaa F Hussein1, Muayad A Hussein2


1Department(s) of Anatomy, College of Veterinary Medicine, University of Baghdad, 2Medical Engineering, University of Technology, Baghdad, Iraq


Habeeb KK, Hussein BF, Hussein MA., In vivo papyrus protein and crab shell bone in dogs, Onl J Vet Res., 27 (10):570-582, 2023. We describe a porous biodegradable bone scaffold without stem cells, implanted in twelve 3-4 year adult mixed breed male dogs weighing ~15kg. Implant consisted of 20 wt.% gelatin from organic bone, 40wt.% dextrin, 20g Al-kharit from papyrus [7], combined with 20g crab shells powder with ~30% protein, ~35% calcium carbonate, ~32% chitin and cellulose-polymer 2% nitrogen, 3% phosphorus, 23% calcium and 1.3% magnesium. The scaffold was cross-linked sterilized by 25Kgy gamma radiation. Micro structure, particle size and crystalline properties were determined by scanning electron microscopy sputtered coated with gold and wide-angle X-ray diffraction. For scaffold implant, a 6 cm incision was made in the skin at craniomedial surface over the radial bone on the proximal third of the left forelimb. A 3 cm critical size defect was created on radial bone by sterile saw for scaffold implant. Microscopy by 120 days showed bone with CaCO3 graphite crystals at 200nm with macro-microspore 25μm-90μm calcium carbonate microcrystal polygonal pores and completely replaced defect.


Key word: Al-kharit, Crab Shell, Tissue Engineering, Bioceramic Scaffold, Dogs.