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Online Journal of Veterinary Research ©
Volume 5: 10-13, 2001. Redacted 2018.
SDS-PAGE proteins in
Corynebacterium pseudotuberculosis toxin (goat).
Mohan P1,
Jayaprakasan V2, Punnoose
KT2, Mini M2
1 Research Associate, Project Directorate on Animal
Disease Monitoring And Surveillance (PD_ADMAS), IAH & VB Campus, Hebbal, Bangalore – 560
024, INDIA. 2Department of Microbiology, College of
Veterinary and Animal Sciences, Kerala Agricultural University, Mannuthy, Thrissur, Kerala, INDIA. *Corresponding
author
SUMMARY
Mohan P, Jayaprakasan V, Punnoose KT, Mini M., SDS-PAGE Proteins in Corynebacterium pseudotuberculosis toxin (goat). Onl J Vet Res., 5: 10-13, 2001. Three toxins were extracted from culture filtrate
(CF), whole cell lysate (WCL) and sodium chloride extract (SCE) from Corynebacterium pseudotuberculosis analysed by SDS-PAGE. Protein fractions with molecular
masses (MM) of 8-200, 20-200 and 19-191KDa were isolated. Toxin fractions from
field isolates and reference strain did not differ; 31.6 and 68KDa bands were
found in the 3 toxins. One fraction (MM 31.6KDa) was identified as
phospholipase D exotoxin which is responsible for dermonecrotoxicity
(antigenic fraction).
Key words: Corynebacterium
pseudotuberculosis; toxin; SDS_PAGE; caprine