©2020-2032 All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to firstname.lastname@example.org publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors.
Online Journal of Veterinary Research ©
Volume 24 (11): 634-645, 2020.
Effect of ion beam irradiation, adenosine and glycine on cordycepin production by Cordyceps militaris fungus.
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Japan
Das SK*, Masuda M, Sakurai A, Sakakibara M., Effect of ion beam irradiation, adenosine and glycine on cordycepin production by Cordyceps militaris fungus, Onl J Vet Res., 24 (11): 634-645, 2020. We boosted cordycepin production from a mutant Cordyeps militaris fungus by ion beam irradiation with adenosine. By proton beam, 30 classes of 8-azaadenine and 28 classes of 8-azaaguanine resistant mutants A63-7, A63-8, A81-2, A81-6, G63-8, G81-3, G82-4 were selected verified by antibacterial production of cordycepin. G81-3 produced 6.84g/l cordycepin compared with controls, 2.45 g/l or 2.79 times higher. We then added 6g/L adenosine in media yielding 8.57 g/l or 28.10% more than controls (6.69 g/l). Consumption of glucose was greatest with 4g/l adenosine but least with 10g/L extending culture time. We also added glycine/yeast extract ratios 40/60, 50/50, 60/40, 70/30, 80/20 and 90/10 or separately as 10, 20 and 30 weight % yielding similar increases in cordycepin. However 90/10 ratio yielded 6.80g/l, 12.40% more than controls (6.05g/l). Ratio of 40/60 weight yeast extract increased glucose consumption and 30 reduced it extending culture time. Our results suggested that ion beam irradiation with adenosine boosted cordycepin production by Cordyceps militaris.
Key words: Ion beam, Cordycepin, Cordyceps militaris, Mutant, Additive.