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Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X


Volume 26 (9): 651-660, 2022.


Functional analysis of Trypanosoma congolense P74 gene by double strand RNA interference.


Waren N. Baticados1 DVM PhD, Noboru Inoue2 DVM PhD, Chihiro Sugimoto3 DVM PhD,

Hediyuki Nagasawa2 DVM PhD and Abigail M Baticados1 DVM


1 College of Veterinary Medicine,University of the Philippines Los Baños, Laguna; 2National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine,  Obihiro, Hokkaido 8555, Japan and 3 Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.




Baticados WN, Inoue N, Sugimoto C,  Nagasawa H, Baticados AM., Functional analysis of Trypanosoma congolense P74 gene by double strand RNA interference, Onl J Vet Res., 26 (9): 651-660, 2022. Trypanosoma congolense RNA interference cell lines were used to determine function of Trypanosoma congolense P74 gene by double strand RNA interference. The present study strongly indicates and further verified the data of the previous research that, P74 gene is stage-specific and the expression is developmentally up-regulated in Trypanosoma congolense bloodstream forms.  The inability to determine the function due to the stage-specific expression of the gene still contributed to the understanding of the nature of the gene and double strand RNA interference in trypanosomes.  The study further stress that T. congolense is still amenable to double strand RNA interference and is an excellent model species to conduct laboratory studies in stage-specific gene expression. 


Key words: Double strand RNA Interference, Trypanosoma congolense, putative ABC1 family protein, procyclic form.