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Online Journal of Veterinary Research

Volume 23 (11):1104-1118, 2019.

Effect of chronic high or low dose verapamil with or without colchicine on serum

and tissue P-glycoprotein and brain histology in Albino mice.


Tayseer Shaker Mahmood, Zainab Ismail Ibrahim, Duraid Abdul-Hadi Abass, Zainab Jamal Mohammad Jawad.


Department(s) of Physiology, Biochemistry & Pharmacology and Pathology, College of Veterinary Medicine, University of Baghdad, Iraq.




Mahmood TS, Ibrahim ZI, Abdul-Hadi Abass D, Mohammad Jawad ZJ., Effect of chronic high or low dose verapamil with or without colchicine on serum and tissue P-glycoprotein and brain histology in albino mice, Onl J Vet Res., 23 (11):1104-1118, 2019. Groups of 7 albino mice each were gavaged 50 and 70 g/kg colchicine with or without 1.5 and 3mg/kg verapamil daily for 90 days. Controls (n=7) were gavaged distilled water. At day 90, heart blood was taken for serum P-glycoprotein determined by ELISA and brain tissue excised for microscopy and P-glycoprotein. Tissue and serum P-glycoprotein decreased 8-24% and 16-28% respectively, with or without verapamil. However, high doses of colchicine with verapamil had no effect on serum P-glycoprotein. High dose colchicine induced mild congestion of blood vessels in meninges and arachinoid plexus, chromatolysis of purkinje cells with mild cytotoxic edema and proliferation, aggregation of oligodendrocytes and mild degeneration of neurons. Low dose verapamil produced lesions similar to those of colchicine but high doses induced severe chromatolysis of purkinje cells. At lower doses, the combined drugs produced hypertrophy and dilation of vessels with congestion, proliferation and aggregation of astrocytes and oligodendrocytes. At higher doses combined drugs micrographs revealed severe chromatolysis, astrocytosis, degeneration, necrosis and de-myelination.


Keywords: Histopathology of brain tissue, colchicine, ELISA test, P-glycoprotein level. Raw data provided.