2021-2033. All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.


OJVRTM

Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

Volume 26 (10): 752-761, 2022.


Long and short-term oviduct cell co-culture and embryo development in ewes.

 

Ehsan Motaghi DVM PhD1, Hooman Bozorgi DVM PhD2, Abbas Shirazi DVM PhD3

 

 

1Young Researchers Club, Azad University, Shahrekord Branch, Shahrekord, 2Department of Clinical Studies, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran.

ABSTRACT

 

Motaghi E, Bozorgi H, Shirazi A., Long and short-term oviduct cell co-culture and embryo development in ewes, Onl J Vet Res., 26 (10): 752-761, 2022. Authors describe effects of long and short-term exposure of oviduct cell co-culture and conditioned medium on ovine embryo development. Fertilized embryos were cultured in oviduct epithelial cell monolayer, oviduct conditioned and control media. For short-term fertilization, embryos were cultured 3 days then transferred to synthetic oviduct fluid cell free media. Embryo development was evaluated by morphological inspection and blastocyst cell numbers counted on day 7. By long-term exposure, fertilized embryos were cultured in OCM and OECM 8 days in 7% oxygen, 5% carbon dioxide and 88% nitrogen. Short-term co-culture of embryos on OECM increased cleavage rate significantly (P<0.05) but had no effect on other variables. Long-term co-culture with OECM significantly increased cleavage rate, total blastocyst rate and blastocyst cell number compared to control group (P<0.05). It was concluded that OECM and OCM in 7% O2, 5% CO2, and 88% N2, have positive effects on sheep embryo development compared to cell free culture.

 

Keywords: ovine, oviduct cell co-culture, embryo, fertilization.


MAIN

 

FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE)