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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 26 (10): 752-761, 2022.
Long and short-term oviduct cell co-culture and embryo development in ewes.
Ehsan Motaghi DVM PhD1, Hooman Bozorgi DVM PhD2, Abbas Shirazi DVM PhD3
1Young Researchers Club, Azad University, Shahrekord Branch, Shahrekord, 2Department of Clinical Studies, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Motaghi E, Bozorgi H, Shirazi A., Long and short-term oviduct cell co-culture and embryo development in ewes, Onl J Vet Res., 26 (10): 752-761, 2022. Authors describe effects of long and short-term exposure of oviduct cell co-culture and conditioned medium on ovine embryo development. Fertilized embryos were cultured in oviduct epithelial cell monolayer, oviduct conditioned and control media. For short-term fertilization, embryos were cultured 3 days then transferred to synthetic oviduct fluid cell free media. Embryo development was evaluated by morphological inspection and blastocyst cell numbers counted on day 7. By long-term exposure, fertilized embryos were cultured in OCM and OECM 8 days in 7% oxygen, 5% carbon dioxide and 88% nitrogen. Short-term co-culture of embryos on OECM increased cleavage rate significantly (P<0.05) but had no effect on other variables. Long-term co-culture with OECM significantly increased cleavage rate, total blastocyst rate and blastocyst cell number compared to control group (P<0.05). It was concluded that OECM and OCM in 7% O2, 5% CO2, and 88% N2, have positive effects on sheep embryo development compared to cell free culture.
Keywords: ovine, oviduct cell co-culture, embryo, fertilization.