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Online Journal of Veterinary Research

 Volume 22 (2): 115-128, 2018.

Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles (Anadara granosa).


Bilung LM1, Radu S2, Bahaman AR3, Rahim RA4, Napis S4, Apun K5, Kqueen CY6, Marlina2Tanil GB2, Nishibuchi M7.


1Department of Biotechnology, Faculty of Engineering and Science, University Tunku Abdul Rahman, 2Department of Food Science, Faculty of Food Science and Technology, 3Faculty of Veterinary Medicine, 4Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences,  6Department of Biomedical Science, Faculty of Health Sciences, University Putra Malaysia,  5Resource Biotechnology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Malaysia and, 7Center for Southeast Asian Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto, 606-8501, Japan.




Bilung LM, Radu S, Bahaman AR, Rahim RA, Napis S, Apun K, Kqueen CY, MarlinaTanil GB, Nishibuchi M. Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles (Anadara granosa), Onl J Vet Res., 22 (2): 115-128, 2018. The incidence of V. parahaemolyticus was evaluated in cockles from coastal Malaysia. Sixty-two of 100 samples were positive for Vibrio parahaemolyticus by PCR. Isolates were screened for tdh and trh genes by PCR assay exhibiting high resistance towards antibiotics. All strains of V. parahaemolyticus exhibited multiple antibiotic resistance (MAR) in the range of 0.58 to 0.94. Isolates (89%) harboured 1-3 plasmids, with sizes ranging from 2.7 to 54 kb. The results suggest that the strains derived from high-risk sources. In addition, no particular plasmid profile was predictive of a particular pattern of antibiotic susceptibility. These findings suggest involvement of seafood especially shellfish and environment in the transmission to humans and may be a source of food-acquired antibiotic resistant bacteria in humans.


Key-Words: Antibiotic, resistance, plasmid, Vibrio para-haemolyticus.