©1994-2018 All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors.

OJVRTM

Online Journal of Veterinary Research©

 Volume 22 (2): 115-128, 2018.

Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles (Anadara granosa).

 

Bilung LM1, Radu S2, Bahaman AR3, Rahim RA4, Napis S4, Apun K5, Kqueen CY6, Marlina2,  Tanil GB2, Nishibuchi M7.

 

1Department of Biotechnology, Faculty of Engineering and Science, University Tunku Abdul Rahman, 2Department of Food Science, Faculty of Food Science and Technology, 3Faculty of Veterinary Medicine, 4Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences,  6Department of Biomedical Science, Faculty of Health Sciences, University Putra Malaysia,  5Resource Biotechnology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Malaysia and, 7Center for Southeast Asian Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto, 606-8501, Japan.

 

ABSTRACT

 

Bilung LM, Radu S, Bahaman AR, Rahim RA, Napis S, Apun K, Kqueen CY, Marlina,  Tanil GB, Nishibuchi M. Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles (Anadara granosa), Onl J Vet Res., 22 (2): 115-128, 2018. The incidence of V. parahaemolyticus was evaluated in cockles from coastal   Malaysia. Sixty-two of 100 samples were positive for Vibrio parahaemolyticus by PCR. Isolates were screened for tdh and trh genes by PCR assay exhibiting high resistance towards antibiotics. All strains of V. parahaemolyticus exhibited multiple antibiotic resistance (MAR) in the range of 0.58 to 0.94. Isolates (89%) harboured 1-3 plasmids, with sizes ranging from 2.7 to 54 kb. The results suggest that the strains derived from high-risk sources. In addition, no particular plasmid profile was predictive of a particular pattern of antibiotic susceptibility. These findings suggest involvement of seafood especially shellfish and environment in the transmission to humans and may be a source of food-acquired antibiotic resistant bacteria in humans.

 

Key-Words: Antibiotic, resistance, plasmid, Vibrio para-haemolyticus.

MAIN

 

FULL-TEXT (SUBSCRIPTION OR PURCHASE ARTICLE)