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OJVRTM

Online Journal of Veterinary Research©

Volume 20(7):486-491, 2016.


Inflammatory markers in experimental caprine coccidiosis.

 

Tadayon Sa, Razavi SMa, Nazifi Sb.

 

aDepartment(s) of Pathobiology, bClinical Studies, School of Veterinary Medicine, Shiraz University,  Iran

 

ABSTRACT

 

Tadayona, S., Razavia, S.M., Nazifib, S., Inflammatory markers in experimental caprine coccidiosis. Onl J Vet Res., 20(7):486-491, 2016. Twenty kid goats aged 2-4 months were given sulfonamide for 5 consecutive days and maintained free of coccidia for 14 days. Ten kids were then infected orally with 5×104 sporulated oocysts E. caprina: 28500, E. ninakohlyakimovae: 14000, and E. arloingi: 7500 in 200 ml distilled water per animal. Another 10 uninfected kids given 200ml saline were used as controls. Blood samples were collected from both groups before infection and at days 3, 7, 14, 21, 28 and 35 post-infection. Fecal oocysts in infected goats appeared at 14 days in 4 goats and 17 days in 6 goats. All infected kids developed dehy­dration, anorexia and depression followed by diarrhea by day 19. Peak fecal shedding occurred at 17-25 days with a mean value of 21×104 oocysts per gram of feces. Serum adenosine deaminase activity (ADA), total (TSA), lipid-bound (LSA) and protein-bound sialic acid (PSA) were significantly higher by 3 to 7 days after infection compared with controls. In particular serum ADA activity increased by 3 days, peaked at 14 days and fell from day 35. The findings suggest the markers may be useful for early signs of coccidia in kid goats.

 

 

Key words: Adenosine deaminase activity, Total sialic acid, Lipid-bound sialic acid, Protein-bound sialic acid, Caprine coccidiosis.


 

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