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OJVRTM
Online
Journal of Veterinary Research©
Volume 16 (1):53-61, 2012. Redacted 2017.
Coagulase gene heterogeneity in bovine mastitic
S. aureus isolates.
Zahra Esmailnezhad DVM PhD, Masoud Haghkhah DVM PhD, Narjes Ghaffari
Department of Pathobiology, School of
Veterinary Medicine, Shiraz University, Shiraz, Iran.
ABSTRACT
Esmailnezhad Z, Haghkhah M, Ghaffari
N., Coagulase gene heterogeneity in bovine mastitic
S. aureus isolates, Onl J Vet Res, 16 (1):53-61,
2012.
S. aureus coagulase
gene is a significant virulence factor whose typing is used to identify S.
aureus genotypes. The present study was conducted to characterize coagulase
gene in bovine mastitic S. aureus isolates.
Milk samples were collected from 40 mastitic cows and
S. aureus isolates were identified by routine biochemical tests
confirmed by species specific PCR to yield products with an expected molecular
size of 1.3 kb. PCR amplification of coagulase gene in all the isolates yielded
single bands with the molecular size of 700 (30%) and 900 bp
(70%). The detection of single bands by coagulase PCR in all isolates showed
that the enzyme were of bovine origin suggesting that there was no human
contamination of bovine mammary glands or milk. Digestion
of coagulase gene PCR products with restriction endonuclease enzymes AluI and Hin61 yielded 3 different RFLP
profiles that indicates a low heterogeneity in the
coagulase gene of the bovine mastitic S.
aureus isolates.