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Online Journal of Veterinary Research©
Volume 22 (4):340-349, 2018.
Effect of clomiphene citrate, metformin, oral contraceptives, spironolactone, and vitamin D
on histomorphology of polycystic ovary in NMRI Mice
Seydi Homeyra MSc1, Azadbakht Mehri PhD*1, Parto Paria PhD1, Bazaz Zohreh PhD2
1Department of Biology, Faculty of Basic Sciences, Razi University, Kermanshah, 2Senior GMP inspector of IFDA, Health Ministry Tehran, Iran.
Homeyra S, Mehri A, Paria P, Zohreh B., Effect of clomiphene citrate, metformin, oral contraceptives, spironolactone, and vitamin D on histomorphology of polycystic ovary in NMRI Mice, Onl J Vet Res., 22 (4):340-349, 2018. We describe effects of clomiphene citrate, metformin, oral contraceptive pills, spironolactone and vitamin D on histo-morphology of polycystic ovaries induced by testosterone in groups of 9 NMRI mice each. Polycystic ovaries were induced in groups of 9 female 3 week old NMRI mice by daily IP injection of 1mg/100g BW testosterone enanthate dissolved in sesame oil for 4 weeks. After induction, mice were treated daily with 1mg/kg clomiphene citrate in drinking water, 250mg/kg metformin, 0.12 mg/kg, ethinyl estradiol subcutaneously and 0.5 mg/kg levonogestril oral contraceptives, 12mg/kg spironolactone or 0.5 µg/kg vitamin D, intraperitoneally. Controls were induced for polycystic ovaries but not treated. Compared with controls, and with exception of mice given oral contraceptives, body and ovarian weights and diameters decreased (p<0.05). Primary and primordial follicles decreased (P < 0.05) except for those given spironolactone. Clomiphene citrate, metformin or oral contraceptives induced highest number of preantral and antral follicles but cystic follicles decreased (p<0.05). In mice given clomiphene citrate, the size of Corpus lutea increased (p<0.05). We find that clomiphene citrate could be used to treat polycystic ovaries in NMRI mice.
Key words: Polycystic ovary syndrome, Clomiphene citrate, Metformin, Oral contraceptives pills, Spironolactone, Vitamin D, Mice.