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OJVRTM
Online Journal of
Veterinary Research©
Volume
24 (2):82-86,
2020.
Sensitivity and
specificity of agar gel (AGPT), immunoelectrophoresis
(CCIEP) and ELISA tests for Cysticercus cellulosae.
S Borkataki, S Islam, DK Sarma, MR Borkakaty and P Goswami.
Department of Parasitology, College of
Veterinary Sciences, Khanapara, Assam Agricultural
University, Guwahati, 781022, India.
ABSTRACT
Borkataki S, Islam S, Sarma DK, Borkakaty MR, Goswami P., Sensitivity and specificity of agar (AGPT), immunoelectrophoresis (CCIEP) and ELISA tests for Cysticercus cellulosae, Onl J Vet Res., 24
(2):82-86, 2020. Sero-diagnosis
of porcine cysticercosis by agar gel precipitation (AGPT), countercurrent immunoelectrophoresis
CCIEP and ELISA of purified fluid, membrane and scolex
antigens of Cysticercus cellulosae is
described. Cisticercosis
was induced in pigs fed bran and molasses mixed with ~1,000,000 eggs of Tania solium. C. cellulosae
infected muscles were excised for cystic fluid, membrane and scolex antigens. Blood was taken 10, 30, 45, 60, 80 and 90th
day after for serum tested
against purified antigen. Antigens were purified by chromatography with 3 peaks
for fluid and 2 for membrane and scolex. Hyper immune sera was raised in NSW rabbits against all the
peaks by 3 booster doses given weekly. In
pig sera, we found no reaction with AGPT and antibody was only detected on the 90th
day after infection with CCIEP or ELISA. However with ELISA tests, cyst fluid, membrane
and scolex antigens reacted positively at any
dilution being 100% specific. Titre of cyst fluid was
1:8000 and membrane and scolex antigen 1:320
suggesting that ELISA was highly sensitive. In experimentally infected pig
serum, a titre of 1:160 could be detected by ELISA at
90th DPI. We find that ELISA was more sensitive and specific and cyst
fluid most immune-reactive to C cellulosae antigen.
Key Words: Serodiagnosis,
Cysticercosis, Pig, AGPT, CCIEP, ELISA.
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