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OJVRTM

Online Journal of Veterinary Research

Volume 24 (2):82-86, 2020.


Sensitivity and specificity of agar gel (AGPT), immunoelectrophoresis (CCIEP) and ELISA tests for Cysticercus cellulosae.

 

S Borkataki, S Islam, DK Sarma, MR Borkakaty and P Goswami.

 

Department of Parasitology, College of Veterinary Sciences, Khanapara, Assam Agricultural University, Guwahati, 781022, India.

 

ABSTRACT

 

Borkataki S, Islam S, Sarma DK, Borkakaty MR, Goswami P., Sensitivity and specificity of agar (AGPT), immunoelectrophoresis (CCIEP) and ELISA tests for Cysticercus cellulosae, Onl J Vet Res., 24 (2):82-86, 2020. Sero-diagnosis of porcine cysticercosis by agar gel precipitation (AGPT), countercurrent immunoelectrophoresis CCIEP and ELISA of purified fluid, membrane and scolex antigens of Cysticercus cellulosae is described. Cisticercosis was induced in pigs fed bran and molasses mixed with ~1,000,000 eggs of Tania solium. C. cellulosae infected muscles were excised for cystic fluid, membrane and scolex antigens. Blood was taken 10, 30, 45, 60, 80 and 90th day after for serum tested against purified antigen. Antigens were purified by chromatography with 3 peaks for fluid and 2 for membrane and scolex. Hyper immune sera was raised in NSW rabbits against all the peaks by 3 booster doses given weekly. In pig sera, we found no reaction with AGPT and antibody was only detected on the 90th day after infection with CCIEP or ELISA. However with ELISA tests, cyst fluid, membrane and scolex antigens reacted positively at any dilution being 100% specific. Titre of cyst fluid was 1:8000 and membrane and scolex antigen 1:320 suggesting that ELISA was highly sensitive. In experimentally infected pig serum, a titre of 1:160 could be detected by ELISA at 90th DPI. We find that ELISA was more sensitive and specific and cyst fluid most immune-reactive to C cellulosae antigen.

 

Key Words: Serodiagnosis, Cysticercosis, Pig, AGPT, CCIEP, ELISA.


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