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OJVRTM
Online Journal of Veterinary Research©
15 (4): 404-413, 2011. Extensively
redacted 2017.
Simple rapid test for ciliary movement in trachea of chicken infected by bronquitis and influenza viruses
Boroomand Z1*, Asasi K2, Hosseinian SA1, Mohammadi
A3, Karimi_Madab M1
1- D.V.M,
Resident in poultry diseases and hygiene, Poultry Research Center, 2- D.V.M, PhD, Professor in Poultry Research
Center, 3- D.V.M, PhD, Asistant Professor in Virology, Department of
Pathobiology, School of Veterinary
Medicine, Shiraz University, Shiraz, Iran
ABSTRACT
Boroomand Z, Asasi K, Hosseinian SA, Mohammadi A, Karimi_Madab M, Simple rapid test for ciliary movement in
trachea of chicken infected by bronquitis and
influenza viruses, Onl
J Vet Res., 15 (4): 404-413, 2011. Mucociliary
clearance is an important defense mechanism for respiratory airways. Authors
describe a method to detect ciliary activity in trachea of chickens infected
with infectious bronchitis virus (IBV) and influenza virus (AIV) with carbon
pigment granules. Groups of ten 21 day old commercial broiler chicks were
inoculated intranasally (IN) with 0.2 ml IBV, 0.2 ml AIV, or
0.2ml IBV and AIV. Controls were inoculated (IN) sterile PBS. In birds given
IBV and/or AIV no pigment appeared in the larinx
whereas in those inoculated with AIV, pigment was present in the larynx.
Results suggest that IBV prevented ciliary movement whereas AIV did not.
Key words: carboniferous pigment granules,
ciliary activity, ciliostasis, IBV, H9N2
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