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OJVRTM
Online Journal of Veterinary Research©
Volume 19(4):
270-281, 2015.
Extraction of rumen α–amylase
with cibacron blue magnetic nanoparticles
Nosrat Allah Ghazvini1, Mohammad Chamani1*, Homayon Ahmad Panahi2,
Ali Asghar
Sadeghi1 and Mehdi Aminafshar1
1 Department(s)
of Animal Science, 2Chemistry, Faculty of Agriculture and
Natural Resources, Tehran Science and Research Branch, Islamic Azad University,
Tehran, Iran.
ABSTRACT
Ghazvini NA, Chamani M, PanahiHA,
Sadeghi AA, Aminafshar M., Extraction
of rumen α–amylase with cibacron blue magnetic
nanoparticles, Onl J Vet Res., 19(4): 270-281, 2015. Magnetic nano-particles (MNPs) were
grafted with allyl 2,3–epoxypropyl ether and coupled with Cibacron blue ligand
to capture α-amylase from clarified ruminal fluid. MNPs
were analyzed by thermogravimetry, transmission
electron microscopy, and Fourier transform infrared spectroscopy. Greatest adsorption efficiency occurred at pH 7, 24°C, and shaking time of 10 minutes. Most efficient desorption
of absorbed active α-amylase occurred in 0.5 M NaCl
solution for 90 minutes shaking (p<0.05). The results suggest that polymerization of Cibacron blue onto MNPs can extract rumen α-amylase.
The purified α-amylase had high activity compared with native α-amylase.
Keywords: Amylase; Magnetic nanoparticles; Clarified rumen fluid;
Adsorption; Desorption.
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