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OJVRTM
Online Journal
of Veterinary Research©
(Including
Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 26 (11): 843-845, 2022.
Effect of dromedary milk on serum lipids and
cholesterol in Wistar rats
Barakat E
Mohamed1, NZ Idam2
1Department
of Chemistry, Faculty of Science, King Faisal University, Kingdom of Saudi
Arabia. 2Faculty of Animal Production, Gezira University-Sudan
ABSTRACT
Mohamed BE, Idam
NZ, Effect of dromedary milk on serum lipids and cholesterol in Wistar rats Onl J Vet Res., 26 (11): 843-845,
2022. The
effect of raw camel milk on plasma lipids in rats fed a cholesterol enriched
diet was investigated. Wistar albino rats were fed a standard
diet (controls), 1% cholesterol or 1% cholesterol with 16 ml/kg raw camel milk
twice daily for 4 weeks. Camel milk did not decrease plasma cholesterol, low
density lipoprotein-c or triglyceride whereas plasma high density liprotein-c was significantly (P<0.05) lower in rats given
1% cholesterol or camel milk.
Key
words: camel milk, cholesterol ,Coronary heart disease
INTRODUCTION
Camel milk is an important
nutrient source for humans (5) but differs from other ruminant milk
having low sugar, cholesterol but high levels of minerals(9) and vitamin C which is ~3X higher than bovine milk(6).
Camel milk contains high amount of poly-unsaturated fatty acids which may lower
serum lipid and reduced cardiovascular disease(8).
Vitamin C can reduce cholesterol biotransformation from bile acids(13).
This vitamin boosts cholesterol 7-α hydroxylase enzyme to catabolizes cholesterol. The effect of raw camel milk on
plasma lipids in hypercholesterolemic rats is
reported.
MATERIALS AND METHODS
Three
groups of 7
adult male Wistar albino rats weighing 85-120 g were housed
in The Medicinal and Aromatic Plant Research Institute,
Sudan fed standard diet acclimatized 7 weeks. Then daily for 4 weeks, controls were
fed standard diet, another group fed a diet with 1% cholesterol to induce
hypercholesterolemia and a group fed 1% cholesterol gavaged
vis gastric tube 16ml/kg raw camel milk twice daily. Blood samples were
collected every two weeks interval during the experimental period, after an
over-night fast. Blood was drawn from the orbital plexus by capillary glass tubes(11) and collected in lithium heparin
containers. The tubes were centrifuged at 5000 rpm for 10 min. for plasma. Plasma
total cholesterol, LDL-c, HDL-c and triglycerides were determined by enzyme
photometric assay (Roche Diagnostic Hitachi 902 Analyzer). Data was
analyzed using complete randomized design to separate the means and standard
error with the aid of a computer package program (SPSS). The significant
differences between groups were evaluated using general linear model of
statistical analysis system (12).
RESULTS
Table (1): Values of plasma lipids profile
of induced hypercholesterolemic
Wistar albino
rats.
Group Parameters |
Controls |
1% cholesterol |
1% cholesterol + milk |
Total
cholesterol (mg/dl) |
81.76 ± 1.98b |
150.00 ±
15.49b |
134.52 ± 14.84a |
HDL-c
(mg/dl) |
47.77 ± 1.83a |
33.30 ±
1.44b |
34.79 ±
1.87b-27% |
LDL-c
(mg/dl) |
27.65 ± 1.80b |
107.40 ±
14.78a |
82.63 ±
13.12a |
Triglycerides
(mg/dl) |
73.29 ± 5.47b |
93.95 ± 6.6a |
90.48 ± 4.87a |
Means (±SE) with the same
row having different superscript small letters are significantly different at
(P<0.05).
Group A: Fed standard diet, Group B:Fed
standard diet with 1% cholesterol, Group C: Fed
standard diet with1% cholesterol and 16 ml/kg Bwt
camel milk
b b ┬ ┬
DISCUSSION
Compared with controls, results
suggest that 16ml/kg raw camel milk in diet had no significant effect on plasma
cholesterol, LDL-c or triglycerides. However, we did find a ~27% reduction (P<0.05)
in plasma HDL-c in rats gavaged camel milk. Our
findings simulate Agrawal et al., (2003)(1) who reported that
oral administration of 500ml raw camel
milk for 3 months did not significantly affect plasma lipid profiles in type 1
diabetic patients whereas Mahamad et al., (2006)(10
found that camel milk have no significant effect on serum lipids gavaged 500ml/kg 4 weeks in type 1 diabetics. Lack of
effect on serum cholesterol may have been due to lower blood vitamin C levels due
to grazing. In contrast, Elayan et al., (2008)(4) reported that 0.5g gariss
supplement given 6 weeks reduced plasma total cholesterol, LDL-c and triglyceride
but had no effect on HDL-c. Suzuki et al, (1991)(14) reported that fermented dairy products reduced
cholesterol(3).
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