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Online Journal of Veterinary Research©
Volume 22 (7):619-628, 2018.
Comparative pathological and molecular study of cholera induced by ingestion of human feces in rats, mice and rabbits.
Muna Sachit Hashim1, Thikra Abdulla Mahmood2, Suad Abdul Kareem Mohammed3, Afaff Abdurrahman4.
1,3,4College of Veterinary Medicine, 2Dep. of Community Medicine, College of Medicine, Kufa University, Iraq.
Hashim MS, Mahmood TA, Kareem Mohammed SA, Abdurrahman A., Comparative pathological and molecular study of cholera induced by ingestion of human feces in rats, mice and rabbits, Onl J Vet Res., 22 (7):619-628, 2018. Ingestion of Vibrio cholera causes acute diarrhea responsible for ~100,000ís deaths per year worldwide and extensively studied in human patients. However, there is limited data on effect of oral infection with human material with vibrio cholera on pathology and immunology and/or DNA damage in reproductive organs in laboratory animals. We infected orally groups of 30 rats, mice and rabbits each with 0.01mg\10gm BW feces with certified Vibrio cholerae from hospitalized patients suffering from cholera, daily for 21 days. Controls (n=30) were given distilled water. As animals died, liver, kidney, lung, brain, intestine and associated lymph nodes, testes and uterus were excised. Twenty percent of infected animals survived and these were also sacrificed for same tissue samples. Histo-immuno-tests showed necrotic foci with hemorrhagic enteritis; liver necrosis; kidney abscesses and brain degeneration with increased expression of CD4 and CD8 markers. Molecular analysis using FISH technique in testes and uterus tissue sections showed DNA damage as mutations in gene TK (11qE2)/XY.
Key words: Cholera; Molecular-Pathogenesis; Rats; Mice; Rabbits.