©1996-2021. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.
Online Journal of Veterinary Research©
Volume 23 (12):1170-1178, 2019.
Chitosan nanoparticle vaccine for avian pathogenic E. coli serotype O78.
Ali Shirazinejada, Abdollah Derakhshandeha, Bahman Abdi-Hachesoob,
*Mohammad Hossein Hosseinic, Nader Ghaleh Golab Behbahanc
aDepartment(s) of Pathobiology, bClinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, cRazi Vaccine and Serum Research Institute, Shiraz, Iran.
Shirazinejad A, Derakhshandeh A, Abdi-Hachesoo B, Hosseini MH, Golab Behbahan NH., Chitosan nanoparticle vaccine for avian pathogenic serotype O78 E. coli, Onl J Vet Res., 23 (12):1170-1178, 2019. Induction of homologous protection against prevalent serotypes of Avian pathogenic E. coli (APEC), is done to reduce losses and mortality. We report results of an inactivated avian pathogenic E. coli (serotype O78) formulated with chitosan nanoparticles in SPF chickens. Groups of 10 three week old SPF chickens were given intramuscular bacterin with chitosan nanoparticles (group 1), saponin (2), montanide oil (3) and PBS (controls). Serum IgG was determined weekly for 7 weeks and then chicks were challenged intravenously E. coli O78. In birds given chitosan or saponin, serum IgG remained ~30-60 lower (P < 0.05) than those injected montanide. However compared with controls those vaccines provided ~1 to 5 fold higher serum IgG. Six of 10 control PBS chicks died by 72h, whereas those immunized, survived.
Key words: Avian colibacillosis, E. coli O78, Chitosan nanoparticles, Saponin, Montanide oil.