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Online Journal of Veterinary Research

Volume 23 (12):1170-1178, 2019.


Chitosan nanoparticle vaccine for avian pathogenic E. coli serotype O78.


Ali Shirazinejada, Abdollah Derakhshandeha, Bahman Abdi-Hachesoob,

*Mohammad Hossein Hosseinic, Nader Ghaleh Golab Behbahanc


aDepartment(s) of Pathobiology, bClinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, cRazi Vaccine and Serum Research Institute, Shiraz, Iran.




Shirazinejad A, Derakhshandeh A, Abdi-Hachesoo B, Hosseini MH, Golab Behbahan NH., Chitosan nanoparticle vaccine for avian pathogenic serotype O78 E. coli, Onl J Vet Res., 23 (12):1170-1178, 2019. Induction of homologous protection against prevalent serotypes of Avian pathogenic E. coli (APEC), is done to reduce losses and mortality. We report results of an inactivated avian pathogenic E. coli (serotype O78) formulated with chitosan nanoparticles in SPF chickens. Groups of 10 three week old SPF chickens were given intramuscular bacterin with chitosan nanoparticles (group 1), saponin (2), montanide oil (3) and PBS (controls). Serum IgG was determined weekly for 7 weeks and then chicks were challenged intravenously E. coli O78. In birds given chitosan or saponin, serum IgG remained ~30-60 lower (P < 0.05) than those injected montanide. However compared with controls those vaccines provided ~1 to 5 fold higher serum IgG. Six of 10 control PBS chicks died by 72h, whereas those immunized, survived.


Key words: Avian colibacillosis, E. coli O78, Chitosan nanoparticles, Saponin, Montanide oil.