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Online Journal of Veterinary Research©

Volume 24(8): 464-470, 2020. .

    Characterization and sequencing of MT-COX1 gene in chickens (Gallus gallus).

 

Seyed Nader Albooshokeh[1], Mojtaba Tahmoorespur[2] and Mohammad Reza Nassiry[3]

 

1Genetics and Animal Breeding, Ferdowsi University of Mashhad International Campus.2Animal Genetics and Biotechnology. Agriculture College. Ferdowsi University of Mashhad.3Agriculture College. Ferdowsi University of Mashhad. Iran.

ABSTRACT

 

Albooshokeh SN, Tahmoorespur M, Nassiry MR., Characterization and Sequencing of MT-COX1 gene  in chickens (Gallus gallus), Onl J Vet Res., 24(8): 464-474, 2020. Two DNA fragments were amplified, cloned to pTZ57R/T vector and transformed into an E. coli - DH5α strain. Recombinant plasmids were then extracted from positive colonies and sequenced. The results of nucleotide sequence indicated maximum similarity with the same sequence in the Gallus Gallus mitochondrial genome reference. Comparing these two sequences identified the presence of 3 mutations. Amino acid sequences of Khorasan’s native chicken had 99% similarity in 100% coverage with reference genome and other sequences with accession numbers YP272100.1, ADB06689.1, AHI87875.1, and YP272087.1. The protein BLAST query suggested the conserved domain cytochrome C, in a family which is part of complex IV. Such mutations may be associated with the evolution of Khorasan wild chickens adaption to environment.

 

KEY WORDS: Mitochondrial – Genome – Gallus Gallus.

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