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OJVRTM
Online Journal of
Veterinary Research©
Volume 9 (1) : 1-5, 2005. Extensively
Redacted 2018.
Electrometric determination of
cholinesterase in chickens exposed to dichlorvos and carbaryl.
Fouad KM, Banan Al-Baggou.
Department of Physiology, Biochemistry and Pharmacology, College
of Veterinary Medicine, University of Mosul, Mosul, Iraq
Abstract
Fouad KM, Banan Al-Baggou., Electrometric determination cholinesterase in chickens exposed to dichlorvos
and carbaryl, Onl J Vet
Res., 9 (1):1-5, 2005. An electrometric method was used for measurement of cholinesterase
(ChE) activity in plasma, brain and liver of 45 days
old domestic chickens of both sexes. ChE assay
solution consisted of 0.2 ml plasma or tissue homogenate, 3ml distilled water,
3ml pH 8.1 phosphate-barbital buffer and 0.12 ml of 7.5% acetylthiocholine
iodide as substrate. One-step incubation was 30min at 37˚C. Normal
reference values for ChE activity (ΔpH/30
min) in plasma of chickens was 0.47, in brain 0.25 and liver 0.21. Oral
administration of 7mg/kg organophosphorus dichlorvos
and 100 mg/kg induced signs of toxicosis
characteristic of cholinergic overstimulation, associated with significant
decreases in plasma ChE from 62 to 29% and increases
in brain from 28 and 65%. The results
suggest that our method detected changes in ChE in
plasma and tissues of broilers exposed to organophosphorus and carbamate
insecticides.
KEY-WORDS: Cholinesterase, Chicken, Dichlorvos,
Carbaryl, Organophosphate, Carbamate