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OJVRTM

Online Journal of Veterinary Research

Volume 9 (1) : 1-5, 2005. Extensively Redacted 2018.


Electrometric determination of cholinesterase in chickens exposed to dichlorvos and carbaryl.

 

Fouad KM, Banan Al-Baggou.

 

Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq

 

Abstract

 

Fouad KM, Banan Al-Baggou., Electrometric determination cholinesterase in chickens exposed to dichlorvos and carbaryl, Onl J Vet Res., 9 (1):1-5, 2005. An electrometric method was used for measurement of cholinesterase (ChE) activity in plasma, brain and liver of 45 days old domestic chickens of both sexes. ChE assay solution consisted of 0.2 ml plasma or tissue homogenate, 3ml distilled water, 3ml pH 8.1 phosphate-barbital buffer and 0.12 ml of 7.5% acetylthiocholine iodide as substrate. One-step incubation was 30min at 37˚C. Normal reference values for ChE activity (ΔpH/30 min) in plasma of chickens was 0.47, in brain 0.25 and liver 0.21. Oral administration of 7mg/kg organophosphorus dichlorvos and 100 mg/kg induced signs of toxicosis characteristic of cholinergic overstimulation, associated with significant decreases in plasma ChE from 62 to 29% and increases in brain from 28 and 65%. The results suggest that our method detected changes in ChE in plasma and tissues of broilers exposed to organophosphorus and carbamate insecticides.

 

KEY-WORDS: Cholinesterase, Chicken, Dichlorvos, Carbaryl, Organophosphate, Carbamate


 

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