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OJVRTM
Online Journal of
Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 28 (11): 812-816, 2024.
Agar precipitation,
immunoelectrophoresis and ELISA tests for Cysticercus cellulosae in
pigs.
Sanku Borkataki,
S Islam, DK Sarma, MR Borkakaty,
P Goswami.
College
of Veterinary Sciences, Khanapara, Assam Agricultural
University, Guwahati, India.
ABSTRACT
Borkataki S, Islam S, Sarma DK, Borkakaty MR, Goswami P., Agar precipitation, immune-electrophoresis and
ELISA tests for Cysticercus
cellulosae, Onl J Vet Res.,
28 (11): 812-816,
2024. We describe sero-diagnosis
of porcine cysticercosis by agar precipitation
(AGPT), counter current immune-electrophoresis (CCIEP) and ELISA tests of
fluids, membranes and scolex antigens of Cysticercus cellulosae
with homologous hyper immune sera. We infected pigs with ~1,000,000 viable eggs
of Tania solium
per os.
Sera from experimentally infected pigs were collected and tested with purified
antigenic components. Antibody in serum was detected at 90 days with CCIEP and
ELISA tests. ELISA was highly sensitive and 100% specific compared with AGPT
and CCIEP with ~75% specificity. We found purified fluid antigens more
immunogenic than membrane or scolex.
Key Words: Agar precipitation,
electrophoresis, ELISA, cysticercus antigens, pig.
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