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OJVRTM
Online Journal of
Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 28 (11): 840-845, 2024.
Agar precipitation,
immunoelectrophoresis and ELISA tests for Cysticercus cellulosae in
pigs.
Sanku Borkataki,
S Islam, DK Sarma, MR Borkakaty,
P Goswami.
College
of Veterinary Sciences, Khanapara, Assam Agricultural
University, Guwahati, India.
ABSTRACT
Borkataki S, Islam S, Sarma DK, Borkakaty MR, Goswami P., Agar precipitation, immune-electrophoresis and
ELISA tests for Cysticercus
cellulosae, Onl J Vet Res.,
28 (11):
840-845, 2024. We describe sero-diagnosis
of porcine cysticercosis by agar precipitation (AGPT),
counter current immune-electrophoresis (CCIEP) and ELISA tests of fluids,
membranes and scolex antigens of Cysticercus cellulosae with homologous hyper immune
sera. We infected pigs with ~1,000,000 viable eggs of Tania solium per os. Sera from experimentally infected
pigs were collected and tested with purified antigenic components. Antibody in
serum was detected at 90 days with CCIEP and ELISA tests. ELISA was highly
sensitive and 100% specific compared with AGPT and CCIEP with ~75% specificity.
We found purified fluid antigens more immunogenic than membrane or scolex.
Key Words: Agar precipitation, electrophoresis,
ELISA, cysticercus antigens, pig.
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