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OJVRTM
Online Journal of Veterinary Research©
Volume 21(9):572-579, 2017.
Effect of Hydralazine on morphine-induced
MCF-7 cell death.
Vahid
Farnia1, Hossein Zhaleh1*, Mohammad Mehdi Zangeneh2,
Akram Zangeneh3, Zhaleh
Shahmoradi1
1Substance
Abuse Prevention Research Center, Kermanshah University of Medical Sciences,
Kermanshah, Iran. Department(s) of 2Clinical Sciences, 3Microbiology, Faculty of
Veterinary Medicine, Razi University, Kermanshah,
Iran.
ABSTRACT
Farnia V, Zhaleh H, Zangeneh
MM, Zangeneh A, Shahmoradi
Z., Effect of Hydralazine on morphine-induced MCF-7 cell death. Onl J Vet Res., 21(9):572-579,
2017.
Effect of hydralazine on morphine induced death of
MCF-7 cells is described. Hydralazine as a vasodilator affects cellular proliferation
and inhibits apoptosis. MCF-7 cells were grown in DMEM culture media, FBS, NEAA, L-glutamine,
penicillin and streptomycin in T-25 cm2 tissue culture flasks. The 7 treatments were: 1mM morphine controls and/or with 1, 10, 100uM or nM
hydralazine. Conditioning media was harvested as hydralazine and 1 day
after plating, cells were washed with PBS. Cell viability was determined by
trypan blue and cell death, by Hoescht/PI stains.
Results showed a dose dependent effect of hydralazine on morphine treated MCF-7
cell viability and death (p<0.05). The effect of different
concentrations of hydralazine on MCF-7 cells indicated a time-dependent
increase in neurite elongation (p<0.05) at 6, 12, 24 and 48 hours,
The findings suggest that hydralazine increased proliferation and growth of
cell lines and may have inhibited cell death induced by morphine. Clinical
trials are required to validate the findings In Vivo.
Keywords: Hydralazine, Morphine, cell
viability, cell death.
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