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Online Journal of Veterinary Research


Volume 23 (2):126-134, 2019.

Protocol for systematic review and meta-analysis of immunity conferred

by liver fluke recombinant protein cathepsin vaccines.


Rama Jayaraj1*, Madurantakam Royam Madhav2, Chellan Kumarasamy3, Rithika Ramesh2, Ritika Shanker2,

Shanthi Sabarimurugan2, Luke Norbury4, David Piedrafita5, Peter Smooker4


1Charles Darwin University, Ellengowan Drive, Darwin, Northern Territory 0909, 2Vellore Institute of Technology (VIT) University, Vellore, India-632014. 3University of Adelaide, North Terrace Campus, Adelaide, South Australia 5005, 4School of Science, RMIT University, Bundoora West Campus, PO Box 71, Bundoora Vic, 3083, 5Faculty of Science and Technology, Federation University Northways Road, Churchill, Australia.




Jayaraj R, Madhav MR, Kumarasamy C, Ramesh R, Shanker R, Sabarimurugan S, Norbury L, Piedrafita D, Smooker P., Protocol for systematic review and meta-analysis of immunity conferred by liver fluke recombinant protein cathepsin vaccines, Onl J Vet Res., 23 (2):126-134, 2019. Fascioliasis is caused by trematodes and is transmittable from animals to humans. Recent findings suggest that recombinant cathepsin proteases may have potential as vaccines against liver fluke. We systematically review immunity conferred by recombinant protein cathepsin vaccines in published animal studies through PubMed, Scopus and Science Direct databases following guidelines in Preferred Reporting Items for Systematic review and Meta-analysis-Protocol [PRISMA-P] [1]. We evaluate immunity conferred by adjuvanted and unadjuvanted Fasciola cathepsin vaccines, vaccination schedule, adjuvants, doses, animal model and immune response type (Th1/Th2). The data will be represented in a narrative review, meta-analysis and flow chart. Results may identify predictive factors and avoid duplication of findings.


Key words: Systematic review, meta-analysis, Liver fluke, Cathepsin, vaccines.