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Online Journal of Veterinary Research©
Volume 22 (5):393-401, 2018.
Genetic variation in a Caspian horse population determined by AFLP Markers.
Hossein Emrani, Saeid Esmaeilkhanian
Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Emrani H, Esmaeilkhanian S., Assessment of genetic variation in a Caspian horse population using AFLP Markers, Onl J Vet Res., 22 (5):393-401, 2018. In order to assess genetic variation within the Caspian horse breed in Iran, we performed an amplified fragment length polymorphism (AFLP) analysis. Thirty two individuals were analysed using five AFLP primer combinations which produced 52 polymorphic bands. Average Neiۥs Genetic heterozygosity, average Uniformity Index of the AFLP fingerprinting pattern within a population and Polymorphism information content (PIC) per marker were determined (calculated assuming the population was at Hardy–Weinberg equilibrium). The highest and the lowest PIC values occurred with E1T2 (0.3224) and E1T1 (0.2846), respectively. The values of average heterozygosity in our study was 0.367 and highest unbiased expected heterozygosity was found in E2T2 (0.4144) while lowest was detected in E1T1 (0.3506). The average number of effective alleles (ne) in primer combination was 1.6. Genetic similarity was calculated according to Jaccard similarity Index and Simple Matching coefficient. Individual dendrogram constructed using the UPGMA analysis clearly distinguished the 32 individuals. The findings suggest that AFLP analysis revealed genetic relationships and variation amongst Caspian horses.
Key words: AFLP, Genetic variation, Caspian horse.
Abbreviations: PIC, Polymorphism information content; PCR, polymerase chain reaction; HWE, Hardy-Weinberg Equilibrium; AFLP, amplified fragment length polymorphism.