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Online Journal of Veterinary Research©
Volume 21(11):728-732, 2017.
Peripheral blood mononuclear cell culture infection with Salmonella typhimurium as carrier for plasmid DNA vaccine.
Azar Motamedi Boroojeni1, Abdollah Derakhshandeh1*, Masoud Haghkhah1, Seyed Amir Hossein Jalali2, Vida Eraghi1
1Department of Pathobiology, School of Veterinary Medicine, Shiraz University, 2Institute of Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan, Iran.
Boroojeni AM, Derakhshandeh A, Haghkhah M, Hossein Jalali SA, Eraghi V., Peripheral blood mononuclear cell culture infection with Salmonella typhimurium as carrier for plasmid DNA vaccine, Onl J Vet Res., 21(11):728-732, 2017. Mononuclear cells in blood are used in vitro for vaccine immunization assay. We describe an optimized peripheral blood mononuclear cells culture infection with Salmonella typhimurium for plasmid DNA vaccine carrier. A eukaryotic expression green fluorescent protein coding sequence plasmid was introduced to an attenuated strain of Salmonella typhimurium. Peripheral blood mononuclear cells were infected with log phase bacteria (MOI). After one hour incubation with bacteria, 100 IU/ml penicillin, 100mg/ml streptomycin and 50mg/ml gentamycin were added to each plate. By 24 hours incubation delivery of plasmid was confirmed by fluorescent microscope.
Keywords: Peripheral blood mononuclear cells, Salmonella typhimurium, bacterial carrier vaccine.