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OJVRTM
Online Journal of Veterinary Research©
Volume 21(11):728-732, 2017.
Peripheral blood mononuclear cell culture
infection with Salmonella typhimurium as
carrier for plasmid DNA vaccine.
Azar
Motamedi Boroojeni1, Abdollah
Derakhshandeh1*, Masoud Haghkhah1,
Seyed Amir Hossein Jalali2, Vida Eraghi1
1Department of
Pathobiology, School of Veterinary Medicine, Shiraz University, 2Institute
of Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan,
Iran.
ABSTRACT
Boroojeni AM, Derakhshandeh A, Haghkhah
M, Hossein Jalali SA, Eraghi
V., Peripheral blood mononuclear cell culture infection with Salmonella
typhimurium as carrier for plasmid DNA vaccine, Onl J
Vet Res., 21(11):728-732,
2017. Mononuclear cells in blood are used in vitro
for vaccine immunization assay. We describe an optimized peripheral blood
mononuclear cells culture infection with Salmonella typhimurium for
plasmid DNA vaccine carrier. A eukaryotic expression green fluorescent protein
coding sequence plasmid was introduced to an attenuated strain of Salmonella
typhimurium. Peripheral blood mononuclear cells were infected with log
phase bacteria (MOI). After one hour incubation with bacteria, 100 IU/ml
penicillin, 100mg/ml streptomycin and 50mg/ml gentamycin were added to each
plate. By 24 hours incubation delivery of plasmid was confirmed by fluorescent
microscope.
Keywords:
Peripheral blood mononuclear cells, Salmonella typhimurium, bacterial
carrier vaccine.