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OJVRTM
Online Journal of Veterinary Research©
Volume 23 (11):1051-1056, 2019..
Effect of
L-carnitine on cryo-tolerance of 16-cell stage bovine
embryos.
Ahmed S Al-Ebady1,
Souhayla O Hussain2, Saad
A Hatif2,
Dhia H Al-Delemy1 and Akbal
O Al-Amery3.
Department(s) of Surgery and Obstetrics,
College(s) of Veterinary Medicine, 1University of Al-Qadisiyah, 2University of Baghdad, 3Department
of ART, Al-Mahmoodiyah Hospital, Ministry of Health,
Iraq.
ABSTRACT
Ahmed S. Al-Ebady, Souhayla O. Hussain, Saad A. Hatif, Dhia H. Al-Delemy and Akbal O. Al-Amery, Effect of L.
Carnitine on cryo-tolerance of 16-cells stage bovine
embryos, Onl J Vet Res., 23 (11):1051-1056, 2019. We report effect of L-carnitine on cryo-tolerance
of 34 vitrified 16-cell stage bovine embryos. Ten non-treated vitrified embryos
were used as controls. Cumulus–oocyte complex (COC) were recovered and graded by
homogenous cytoplasm surrounded by one or more layer of cumulus cells matured
in TCM-199 supplemented with 10% FCS (controls) or 10% FCS and 0.6 mg/ml L-
Carnitine. After 24 hours, mature oocytes were fertilized in frozen-thawed straws and 18-20h
fertilization, zygotes denuded cultured in CR1aa medium supplemented 0.6 mg/ml L.Carnitine. Embryos were vitrified in 0.25ml straws with
holding, equilibration A and B, vitrification and
dilution mediums vitrified in straws immersed in liquid nitrogen for 1 week. For
warming and re-culturing, straws were held for 10 seconds at room temperature
(20-24ºC), then placed in a water-bath at (20-24ºC) and embryos re-cultured in
TCM-199 supplemented with 1% BSA, and assessed for further development after 24
hours. Results show that of 34 embryos vitrified with L-carnitine, 14 were
viable at warming and 8 twenty four hours later whereas only 2 control embryos
survived at warming and none by 24h (P<0.01). We find that L-Carnitine
enhanced survival of vitrified bovine embryos after warming.
Key words: IVF, bovine embryos vitrification, L. Carnitine.