©1996-2013
All
Rights Reserved. Online Journal of Bioinformatics . You may
not store these pages in
any form except for your own personal use. All other usage or
distribution is
illegal under international copyright treaties. Permission to
use any of these pages in any other way besides the before
mentioned must be
gained in writing from the publisher. This article is
exclusively
copyrighted in its entirety to OJB publications. This article
may be copied
once but may not be, reproduced or re-transmitted without the
express
permission of the editors.
This
journal satisfies the
refereeing requirements (DEST) for the Higher Education
Research Data
Collection (Australia). Linking:To link to this page or any pages linking
to this page you
must link directly to this page only here rather than put up
your own page.
OJBTM
Online
Journal of Bioinformatics ©
Volume 14 (1): 56-65, 2013
Sequence
informatics of carbonic anhydrase from different source organisms
Vivek Dhar Dwivedi,
Shikha Arora,
Jyoti
Sharma, and Amit Pandey*
Forest Pathology Division, Forest Research
Institute, Dehradun,
India
ABSTRACT
Dwivedi VD, Arora S, Sharma
J, Pandey A Sequence
Informatics of Carbonic
anhydrase Enzyme from Different Source organisms, Online J Bioinform.,
14 (1): 56-65, 2013. Carbonic
anhydrases
are widely distributed among bacteria, fungi, plants and
animals. Forty
full length amino acid
sequences of the enzyme were subjected to multiple sequence
alignment (MSA),
motif identification, domain identification, individual amino
acid composition
and phylogenetic analysis. MSA revealed that 1 alanine and glutamic
acid
residues were semi-conserved in all sequences while 1 proline
and glycine residues were identically conserved in all
sequences of fungi. Two
major sequence clusters were obtained by phylogenetic
analysis. One cluster
contains 10 species of animals, 9 fungi, 2 bacteria and 1
plant, whereas another
cluster contains 9 species of plants, 8 bacteria, and 1 fungus
but no animal
species were found in this cluster. Amino acid composition
results revealed
that the average frequency of alanine residue was 8.99% which
was very high compared
with the average frequency of other amino acids in all
analyzed sequences. Fifteen
amino acids Motif (QSPKAMVLACSDSRV)
was found in all bacterial and plant
sources. In addition,
some motifs belonging to Pro_CA
and Carb_anhydrase
domain family unique
to their groups, were also
identified.
Keywords: Carbonic anhydrase, MSA,
Phylogenetic analysis, Conserved regions, Motifs, Domains.