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OJVRTM
Online Journal of Veterinary Research©
Volume
24 (5):271-280, 2020.
Effect of Cannabis
sativa on brain tissue and memory in male Wistar rats.
Farshid Parsa1, Seyed
Ebrahim Hoseini1, Davood
Mehrabani2,3,4,5,
Seyedeh Sara Hashemi3, Amin
Derakhshanfar6
1Department
of Biology, Shiraz Branch, Islamic Azad University, Shiraz, 2Stem
Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz,
3Burn and Wound Healing Research Center, Shiraz University of
Medical Sciences, Shiraz,4Center of Comparative and Experimental
Medicine, Shiraz University of Medical Sciences, Shiraz, 5Li Ka Shing Center for Health Research and Innovation, University
of Alberta, Edmonton, AB, Canada, 6Diagnostic Laboratory
Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz
University of Medical Sciences, Shiraz, Iran *Correspondence: Seyed Ebrahim Hoseini,
Ph.D., Professor of Department of Biology, Shiraz Branch, Islamic Azad
University, Shiraz, Iran, Tel: +98-09171183917, email: mehrabad@outlook.com
ABSTRACT
Parsa F, Hoseini SE, Mehrabani D, Hashemi SS, Derakhshanfar A., Effect of Cannabis sativa
on brain tissue and memory in male Wistar rats, 24
(5):271-280, 2020. Cannabis at high
doses has been used for psychosis and seizures. We evaluate histopathology of brain
tissue and effect on spatial memory and Y maze entry in Wistar
rats given 2mg/kg subcutaneous (SC) Cannabis sativa
daily for 21 days. Groups of 15 rats each were given 2 mg/kg cannabis in 0.6 mL
for 21 days. Controls were given SC 0.6ml ethanol or saline. Rats were
sacrificed at 7, 14 and 21 days to evaluate histopathology of brain tissue. Memory
was assessed for 3 weeks by mean spatial assessment (%)
and number of entries in a Y-maze. Compared with saline controls we found 31-23% less
spatial memory and up to 39% reduced Y maze entry (P 0.01) in those given Sativa cannabis. In meninges, 7-14 days after cannabis
administration we observed hemorrhage and hypertrophy whereas by 21 day, we observed, hyperemia, extravasation, hemorrhage, laminar
necrosis, gliosis and granuloma in both meninges and cerebellum.
Key words: Cannabis sativa, Nervous system, Brain, Memory, Rat.
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