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Online Journal of Veterinary Research©
Volume 24 (5):271-280, 2020.
Effect of Cannabis sativa on brain tissue and memory in male Wistar rats.
Farshid Parsa1, Seyed Ebrahim Hoseini1, Davood Mehrabani2,3,4,5,
Seyedeh Sara Hashemi3, Amin Derakhshanfar6
1Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, 2Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, 3Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz,4Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, 5Li Ka Shing Center for Health Research and Innovation, University of Alberta, Edmonton, AB, Canada, 6Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran *Correspondence: Seyed Ebrahim Hoseini, Ph.D., Professor of Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran, Tel: +98-09171183917, email: firstname.lastname@example.org
Parsa F, Hoseini SE, Mehrabani D, Hashemi SS, Derakhshanfar A., Effect of Cannabis sativa on brain tissue and memory in male Wistar rats, 24 (5):271-280, 2020. Cannabis at high doses has been used for psychosis and seizures. We evaluate histopathology of brain tissue and effect on spatial memory and Y maze entry in Wistar rats given 2mg/kg subcutaneous (SC) Cannabis sativa daily for 21 days. Groups of 15 rats each were given 2 mg/kg cannabis in 0.6 mL for 21 days. Controls were given SC 0.6ml ethanol or saline. Rats were sacrificed at 7, 14 and 21 days to evaluate histopathology of brain tissue. Memory was assessed for 3 weeks by mean spatial assessment (%) and number of entries in a Y-maze. Compared with saline controls we found 31-23% less spatial memory and up to 39% reduced Y maze entry (P 0.01) in those given Sativa cannabis. In meninges, 7-14 days after cannabis administration we observed hemorrhage and hypertrophy whereas by 21 day, we observed, hyperemia, extravasation, hemorrhage, laminar necrosis, gliosis and granuloma in both meninges and cerebellum.
Key words: Cannabis sativa, Nervous system, Brain, Memory, Rat.