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OJVRTM
Online
Journal of Veterinary Research©
Volume 18(9): 718-726, 2014.
Effects of
curcumin and ethanolic turmeric extract on growth and
proliferation of cancerous cells (4T1)
Hooman Yazdanpour
1, Gholamali Jelodar 1, Saeed Nazifi
2
Departments of Basic Sciences1
and Clinical
Studies2, School of Veterinary Medicine, Shiraz University, Shiraz,
Iran
ABSTRACT
Yazdanpour H, Jelodar
G, Nazifi S., Effects of curcumin and ethanolic turmeric extract on growth and proliferation of
cancerous cells (4T1), Onl J Vet Res., 18(9):
718-726, 2014. Anti-carcinogenic properties of curcumin in
animals have been demonstrated by inhibition of tumor initiated induced
carcinogens and phorbol esters. Anti-tumor effects of
curcumin in cancers of the colon, forestomach and breast have been published
recently. We report the effect of different concentrations of curcumin and ethanolic turmeric extract (ETE) on growth of 4T1
cells. 4T1 cells were grown in RPMI
media with 0, 25, 50 and 75 µM/Lit pure curcumin and ETE. The percentage of
viable cells (by MTT) was then assessed. Compared with controls of 0 µM and 25
and 75 µM curcumin or ETE, 68 and 40%, and 82 and 50% of cells respectively,
survived. Total antioxidant activity (TAC) showed significant differences at
any concentration of curcumin and ETE. The highest TAC value was with curcumin
75µM (1.9 mM/l); and the lowest value for ETE 25µM
(1.1 mM/l). The findings suggest that curcumin and
ETE could have an inhibitory effect on growth of 4T1 cells, possibly due to
changes in TAC.
KEY WORDS Cancer cells,
curcumin, turmeric extract, anti-oxidants.
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