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OJVRTM
Online Journal of Veterinary Research©
Volume 22 (8):675-680, 2018.
Effect
of cannabidiol on tumor size and blood
oxidation markers in mice injected CT36 colon cancer cells.
Masoumeh
Honarmand1, Fatemeh Namazi2, Ali Mohammadi2,
Saeed Nazifi1*.
1Department(s) of Clinical
Studies, 2Pathobiology, School of Veterinary Medicine, Shiraz
University, Shiraz, Iran.
ABSTRACT
Honarmand M, Namazi
F, Mohammadi A, Nazifi S., Effect
of cannabidiol
on tumor size and oxidation markers in mice injected CT36 colon cancer cells. Onl J Vet Res., 22
(8):675-680, 2018. Colon cancer is the third most common human malignancy and a main
cause of death worldwide. Authors describe effects of cannabidiol
(CBD) on angiogenesis and cell death in mice with experimental colon cancer induced
by injection of CT26 cell line. ~1×106 cells dissolved in 2ml PBS were
injected IP in left flank of mice. Five groups of 10 BALB/c mice each were normal
(without cancer cells), CT26 cancer and vehicle tween 80 controls, or injected
IP 3 times weekly for 7 weeks with 1 or 5mg/kg CBD, when tumor sizes had
reached 200-300mm3. Tumor size (mm3) was measured weekly.
At end of study, blood glutathione peroxidase, glutathione reductase,
superoxide dismutase and malondialdehyde (MDA) were
determined. By the 5th week, tumors had
grown less (P < 0.05) in mice given CBD with least in those given 5 mg/kg
and by 6-7th weeks in those given 1mg/kg compared with cancer and
vehicle controls. By 7 weeks we found significantly (P 0.05) higher blood
antioxidant but lower MDA in mice given CBD compared with cancer or vehicle
controls. Results suggest that cannabidiol may increase
blood antioxidant enzymes and inhibit colon cancer cell proliferation in mice.
Keywords:
Cannabidiol, Colon cancer, Oxidative stress. Raw data
files provided.
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