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OJVRTM

 Online Journal of Veterinary Research

Volume 22 (8):675-680, 2018.


Effect of cannabidiol on tumor size and blood oxidation markers in mice injected CT36 colon cancer cells.

 

Masoumeh Honarmand1, Fatemeh Namazi2, Ali Mohammadi2, Saeed Nazifi1*.

 

1Department(s) of Clinical Studies, 2Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

 

ABSTRACT

 

Honarmand M, Namazi F, Mohammadi A, Nazifi S., Effect of cannabidiol on tumor size and oxidation markers in mice injected CT36 colon cancer cells. Onl J Vet Res., 22 (8):675-680, 2018. Colon cancer is the third most common human malignancy and a main cause of death worldwide. Authors describe effects of cannabidiol (CBD) on angiogenesis and cell death in mice with experimental colon cancer induced by injection of CT26 cell line. ~1106 cells dissolved in 2ml PBS were injected IP in left flank of mice. Five groups of 10 BALB/c mice each were normal (without cancer cells), CT26 cancer and vehicle tween 80 controls, or injected IP 3 times weekly for 7 weeks with 1 or 5mg/kg CBD, when tumor sizes had reached 200-300mm3. Tumor size (mm3) was measured weekly. At end of study, blood glutathione peroxidase, glutathione reductase, superoxide dismutase and malondialdehyde (MDA) were determined. By the 5th week, tumors had grown less (P < 0.05) in mice given CBD with least in those given 5 mg/kg and by 6-7th weeks in those given 1mg/kg compared with cancer and vehicle controls. By 7 weeks we found significantly (P 0.05) higher blood antioxidant but lower MDA in mice given CBD compared with cancer or vehicle controls. Results suggest that cannabidiol may increase blood antioxidant enzymes and inhibit colon cancer cell proliferation in mice.

 

Keywords: Cannabidiol, Colon cancer, Oxidative stress. Raw data files provided.


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