1994-2021. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors.


Online Journal of Veterinary Research

Volume 23 (8):763-775, 2019.


Effect of calciferol, calcium and metformin on type 2 diabetes mellitus in male rabbits.


Warda Theab Farhan and Duraid A Abbas.


Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Baghdad, Bagdad, Iraq




Farhan WT, Abbas DA., Effect of calciferol, calcium and metformin on type 2 diabetes mellitus in male rabbits, Onl J Vet Res., 23 (7):763-775, 2019. Type 2 Diabetes mellitus was induced in rabbits with 120mg/kg alloxan and 50mg/kg nicotinamide injected intraperitoneally (IP). The condition was confirmed by fasting blood glucose (FBS) of ≥ 200 mg/dl and insulin resistance >3mg/dL at 2 weeks. We then intubated groups of 5 rabbits each with 100IU Vitamin D (VitD) with or without 500mg/kg calcium gluconate (Ca) or 11mg/kg metformin daily for 30 days. Controls consisted of 5 diabetic and 5 normal rabbits given distilled water. We assessed clinical status and body weights, serum FBS, insulin, insulin resistance, interlukein-6, calcium and VitD levels before or 14 and 30 days after induction of diabetes. Compared with controls (P < 0.05), by 30 days body weights declined 28-30% in diabetic rabbits but less in those given VitD (-11%). By 14 days FBS increased ~56% in diabetic rabbits, but by 30 days all treated rabbits had normal FBS. Serum insulin declined 15-38% in all groups except for those given VitD. At 14 days, we found large rises (~40%) in insulin resistance, but by day 30, rabbits given VitD had normal values. Similar results were found with Interleukin-6. At day 14 there were large declines in serum Ca -68% in treated or not diabetic rabbits but by 30 days not in those given VitD. Our findings suggest that Vitamin D supplementation ameliorates effects of type 2 diabetes mellitus in rabbits.


Key word : Diabetes, Metformin, Vitamin D, Calcium, interleukin-6, insulin resistance. Raw data provided after reference list.