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Online Journal of Veterinary Research©
Volume 22 (4):253-257, 2018.
Growth of fungi and bacteria in sterile and non-sterile Theobroma cacao and Linum usitatissmum media
Maysoon S Abbas1, Shaimaa N Yassein2
1Zoonosis unit, 2Department of Microbiology, College of Veterinary Medicine, University of Baghdad
Abbas MS, Yassein SN., Growth of fungi and bacteria in sterile and non-sterile Theobroma cacao and Linum usitatissmum media, Onl J Vet Res., 22 (4):253-257, 2018. We describe Theobroma cacao and Linum usitatissmum as novel media compared with sabourad dextrose (SDA), MacConckey, manitol and kanamycin agars for cultivation of penicillium spp., A. niger, A. fumigatus, T. mentagrophyt, T. rubrum, candida albicans klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli and Streptococcus pneumoniae incubated at 25-37˚C. Cacao and flaxseed media were prepared by suspending dehydrated powders in agar and distilled water, and boiled and sterilized. To determine effect of temperature on media, some samples were not autoclaved. Results showed that fungi grew in T cacao and L usitatissmum compared with other media whereas bacteria did not. We find that Theobroma cacao and Linum usitatissmum could be used as alternative culture supplement for fungal growth.
Keywords: fungi, bacteria, modified media, Theobroma cacao, Linum usitatissmum.