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 OJVRTM

Online Journal of Veterinary Research  

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 28 (1): 37-42, 2024.


Inhibition of infectious bursal disease virus by Withania somnifera

 

Manali Pant, Umapathi V, Tanuj Ambwani, Rashmi Singh, Anil Kumar.

 

Department Of Biotechnology, Graphic Era University,Dehradun, IVRI, Palampur, Veterinary Biochemistry, ,G.B.P.U.A&T,Pantnagar, Microbiology, Mathura University, Department Of Molecular Biology and Genetic Engineering G.B.P.U.A&T,Pantnagar, India.

 

ABSTRACT

 

Pant M, Umapathi V, Ambwani T, Singh R, Kumar A., Inhibition of infectious bursal disease virus by Withania somnifera, Onl J Vet Res., 28 (1): 37-42, 2024. Infectious bursal disease is a notifiable, highly contagious viral disease of chickens mostly 3-6 weeks of age. Effect of Withania somnifera (WS) on replication of infectious bursal disease (IDB) virus is described. Different passages of UABz-2 strain of IBD virus were cultured through chicken embryo fibroblast. Primer set (23 mer) was used for amplification of 248 bp fragment of hypervariable region of VP2 gene of IBDV6. For IBDV growth and inhibition by WS, virus titration was performed. Antiviral activity of extract was determined by treating virus infected CEF cells with WS and cytopathicity observed at 24 hrs interval for three days. Reading CPE was recorded and titer calculated. Hydro alcoholic extract of Withania somnifera inhibited IBD virus 99.9 % at a concentration of 25g/ml in cytopathic inhibition assays. Findings were supported by RT-PCR.

 

Keywords: Infectious Bursal Disease Virus, Ashwagandha, Hydroalcoholic extract, RT-PCR.


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