2023-2035 All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the  before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR.This article may be copied once but may not be, reproduced or  re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.


Online Journal of Veterinary Research  

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X


Volume 28 (1): 37-42, 2024.

Inhibition of infectious bursal disease virus by Withania somnifera


Manali Pant, Umapathi V, Tanuj Ambwani, Rashmi Singh, Anil Kumar.


Department Of Biotechnology, Graphic Era University,Dehradun, IVRI, Palampur, Veterinary Biochemistry, ,G.B.P.U.A&T,Pantnagar, Microbiology, Mathura University, Department Of Molecular Biology and Genetic Engineering G.B.P.U.A&T,Pantnagar, India.




Pant M, Umapathi V, Ambwani T, Singh R, Kumar A., Inhibition of infectious bursal disease virus by Withania somnifera, Onl J Vet Res., 28 (1): 37-42, 2024. Infectious bursal disease is a notifiable, highly contagious viral disease of chickens mostly 3-6 weeks of age. Effect of Withania somnifera (WS) on replication of infectious bursal disease (IDB) virus is described. Different passages of UABz-2 strain of IBD virus were cultured through chicken embryo fibroblast. Primer set (23 mer) was used for amplification of 248 bp fragment of hypervariable region of VP2 gene of IBDV6. For IBDV growth and inhibition by WS, virus titration was performed. Antiviral activity of extract was determined by treating virus infected CEF cells with WS and cytopathicity observed at 24 hrs interval for three days. Reading CPE was recorded and titer calculated. Hydro alcoholic extract of Withania somnifera inhibited IBD virus 99.9 % at a concentration of 25g/ml in cytopathic inhibition assays. Findings were supported by RT-PCR.


Keywords: Infectious Bursal Disease Virus, Ashwagandha, Hydroalcoholic extract, RT-PCR.