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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 27 (9): 539-545, 2023.
Effect of Coxiella burnetii on egg embryo development and pathology.
Norina L2, Noordin MM1, Sabri MY1, Goh MY1, Zamri-Saad M1, Sarenasulastri AB2, Azizul MO2, Norsyahril A2, Krishnan M2, Salmeah AR2, Cheah CP2.
1Faculty of Veterinary Medicine, Univerity Putra Malaysia 43400 Serdang, Selangor, 2Malaysian Animal Health Laboratory (MALH), Bukit Tengah 14000 Bukit Mertajam, Penang.
Norina L, Noordin MM, Sabri MY, Goh MY, Zamri-Saad M, Sarenasulastri AB, Azizul MO, Norsyahril A, Krishnan M, Salmeah AR, Cheah CP., Effect of Coxiella burnetii on egg embryo development and pathology, Onl J Vet Res., 27 (9): 539-545, 2023. Tissue samples were taken from goats with history of abortion tested for Coxiella burnetti by stain smear confirmed by PCR. Six samples of 15 embryonated chicken eggs each, were inoculated with 10-4, 10-5, 10-6, 10-7, 10-8 and 10-9 Coxiella burnetti. Control eggs were inoculated PBS. Embryos were harvested by 10 days for lesion microscopy, Gimenez stain coccobacilli confirmed by immunochemistry. Embryos in eggs inoculated 10-4 Coxiella burneti started to die by day 2 and by day 4 with 10-5 bacteria. There was no mortality observed from day 4 to 10. We found no chorion-allantoic membrane lesions by day 2 to 5. Controls had clear chorion with intact normal blood vessels. In contrast by day 5, those inoculated 10-4 Coxiella presented cloudy membranes and albumin with white spotted foci attached to the chorionic membrane and collapsed blood vessels. Our results were confirmed by immunohistochemistry being very sensitive to antigen. We find that inoculation of embryonated SPF egg with Coxiella burnetti could avoid use of tissue or animals if carried out in Biohazard safety cabinet Class II (Esco) safety protocol.
Key words: Coxiella burnetii, IHC, SPF embryonated egg, goat